数字农科院2.0

Development And Detection Application Of M.onoclonal Antibodies Against Zucchini Y ellow Mosaic Virus

文献类型： 外文期刊

作者： Zhou Xue-ping;Zhou Xue-ping;Zhang Ming-hao;Chen Zhe;Wu Jian-xiang

作者机构：

关键词： Zucchini yellow mosaic virus; monoclonal antibody; ACP-ELISA; dot-ELISA; tissue blot-ELISA; DAS-ELISA; IC-RT-PCR

期刊名称： JOURNAL OF INTEGRATIVE AGRICULTURE

ISSN： 2095-3119

年卷期： 2017 年 16 卷 1.0 期

页码：

收录情况： JCR（2021版） ; CSCD（2020-2021年度） ; 农林核心（2020版） ; 科技核心（2020版）

摘要： Aphid-borne Zucchini yellow mosaic virus (ZYMV) is one of the most economically important viruses of cucurbitaceous plants. To survey and control this virus, it is necessary to develop an efficient detection technique. Using purified ZYMV virion and the conventional hybridoma technology, three hybridoma cell lines (16A11, 5A7 and 3B8) secreting monoclonal antibodies (MAbs) against ZYMV Zhejiang isolate were obtained. The working titers of the ascitic fluids secreted by the three hybridoma cell lines were up to 10(-7) by indirect enzyme-linked immunosorbent assay (ELISA). All MAbs were isotyped as IgG1, kappa light chain. Western blot analysis indicated that the MAb 3B8 could specifically react with the coat protein of ZYMV while MAbs 5A7 and 16A11 reacted strongly with a protein of approximately 51 kDa from the ZYMV-infected leaf tissues. According to this molecular weight, we consider this reactive protein is likely to be the HC-Pro protein. Using these three MAbs, we have now developed five detection assays, i.e., antigen-coated-plate ELISA (ACP-ELISA), dot-ELISA, tissue blot-ELISA, double-antibody sandwich ELISA (DAS-ELISA), and immunocapture-RT-PCR (IC-RT-PCR), for the sensitive, specific, and easy detection of ZYMV. The sensitivity test revealed that ZYMV could be readily detected respectively by ACP-ELISA, dot-ELISA, DAS-ELISA and IC-RT-PCR in 1:163840, 1:2560, 1:327680 and 1:1310720 (w/v, g mL(-1)) diluted crude extracts from the ZYMV-infected plants. We demonstrated in this study that the dot-ELISA could also be used to detect ZYMV in individual viruliferous aphids. A total of 275 cucurbitaceous plant samples collected from the Zhejiang, Jiangsu, Shandong and Hainan provinces, China, were screened for the presence of ZYMV with the described assays. Our results showed that 163 of the 275 samples (59%) were infected with ZYMV. This finding indicates that ZYMV is now widely present in cucurbitaceous crops in China. RT-PCR followed by DNA sequencing and sequence analyses confirmed the accuracy of the five assays. We consider that these detection assays can significantly benefit the control of ZYMV in China.

分类号：

  • 相关文献

[1]Monoclonal Antibody-Based Serological Detection Methods F.or Wheat Dwarf Virus. Zhang, MH, Chen, R, Zhou, XP, Wu, JX. 2018

[2]Monoclonal antibody-based serological detection of potato virus M in potato plants and tubers. Wu Jian-xiang,Zhou Xue-ping,Zhou Xue-ping,Gao Yan-ling,He Wan-qin,Zhang Yu,Qian Ya-juan,Wang Ya-qin. 2020

[3]Feeding Of Riptortus Pedestris On S.oybean Plants, The Primary C ause Of Soybean Staygreen Syndrome In The Huang-Huai-Hai River Basin. Li, K, Zhang, XX, Guo, JQ, Penn, H, Wu, TT, Li, L, Jiang, H, Chang, LD, Wu, CX, Han, TF. 2019

[4]Development of bottle gourd lines resistant to zucchini yellow mosaic virus using ethyl methanesulfonate mutagenesis. Asma Mohammed Saeed Al-Kubati,Baoshan Kang,Liming Liu,Aqleem Abbas,Qinsheng Gu. 2021

[5]Zucchini yellow mosaic virus-induced hypersensitive response is associated with pathogenesis-related 1 protein expression and confers resistance in watermelon. Xiaoyuan Hao,Fengnan Liu,Liming Liu,Huijie Wu,Zhiling Liang,Wei Zhao,Yue Wang,Qinsheng Gu,Baoshan Kang. 2024

[6]A Bifunctional Nuclease Promotes the Infection of Zucchini Yellow Mosaic Virus in Watermelon by Targeting P3. Baoshan Kang,Lifeng Liu,Liming Liu,Mei Liu,Huijie Wu,Bin Peng,Zhiling Liang,Fengnan Liu,Yaoxing Zang,Qinsheng Gu. 2024

[7]Monoclonal antibody-based serological detection of rice stripe mosaic virus infection in rice plants or leafhoppers. Guo, Liqian,Zhou, Xueping,Wu, Jiayu,Chen, Rui,Hong, Jian,Zhou, Xueping,Wu, Jianxiang. 2020

[8]Monoclonal Antibody-Based Serological Assays For D.etection Of Potato Virus S In Potato Plants. Song, Ge,Liu, Yong,Zhou, Xue-ping,Xie, Yan,Wu, Jia-yu,Qian, Ya-juan,Zhou, Xue-ping,Wu, Jian-xiang. 2017

[9]Preparation And Application Of Monoclonal A.ntibody To Sugarcane (Saccharum L . Spp. Hybrids) Sop5Cs Protein. Li, Y. R.,Wang, L. R.,Yang, L. T.,Xing, Y. X.,Huang, X.,Li, Y. R.,Huang, X.,Li, Y. R.,Li, J.,Huang, X.. 2019

[10]Evaluation Of The Protective Immunity Of Riemerella Anatipestifer Ompa. Xu, XX, Xu, YH, Miao, S, Jiang, P, Cui, JS, Gong, YS, Tan, PP, Du, XL, Islam, N, Hu, QH. 2020

[11]Development Of An Indirect Competitive E.lisa For The Detection O f Acenaphthene And Pyrene. Qiao, B, Li, YS, Meng, XY, Sun, Y, Hu, P, Lu, SY, Ren, HL, Liu, ZS, Zhou, Y. 2017

[12]Determination Of Deoxynivalenol By Elisa A.nd Immunochromatographic Strip Assay B ased On Monoclonal Antibodies. Yon, TT, Zhang, Q, Wang, D, Li, PW, Tang, XQ, Zhang, W. 2019

[13]Expression And Characterization Of A R.ecombinant Porcinized Antibody Against T he E2 Protein Of Classical Swine Fever Virus. Chen, SC, Li, S, Sun, HM, Li, YF, Ji, SW, Song, K, Zhang, LK, Luo, YZ, Sun, Y, Ma, JF, Liu, PH, Qiu, HJ. 2018

[14]Identification And Characterization Of A N.ovel B-Cell Epitope On A leutian Mink Disease Virus Capsid Protein Vp2 Using A Monoclonal Antibody. Lu, TF, Wang, YZ, Ge, JW, Ma, Q, Yan, WZ, Zhang, YY, Zhao, LL, Chen, HY. 2018

[15]Neutralization Of Genotype 2 Porcine E.pidemic Diarrhea Virus Strains B y A Novel Monoclonal Antibody. Liu, JB, Shi, HY, Chen, JF, Zhang, X, Ji, ZY, Yuan, J, Shi, D, Cao, LY, Zhu, XD, Dong, H, Wang, XB, Zhang, JL, Feng, L. 2017

[16]An Ultrasensitive Gray-Imaging-Based Quantitative Immunochromatographic D.etection Method For Fumonisin B -1 In Agricultural Products. Tang, XQ, Li, PW, Zhang, ZW, Zhang, Q, Guo, JN, Zhang, W. 2017

[17]Identification Of A Serotype-Independent Linear E.pitope Of Foot-And-Mouth Disease V irus. Yang, BL, Wang, MX, Liu, WM, Xu, ZQ, Wang, HW, Yang, DC, Ma, WG, Zhou, GH, Yu, L. 2017

[18]Identification Of A Conformational Neutralizing E.pitope On The Vp1 P rotein Of Type A Foot-And-Mouth Disease Virus. Liu, WM, Yang, BL, Wang, MX, Wang, HW, Yang, DC, Ma, WG, Zhou, GH, Yu, L. 2017

[19]The Simultaneous Detection of Multiple Antibiotics in Milk and Pork Based on an Antibody Chip Biosensor. Xiao J., Wei N., Wu S., Li H., Yin X., Si Y., Li L., Peng D.. 2022

[20]Characterization Of Monoclonal Antibodies Against S.igma A Protein And C ross-Reactive Epitope Identification And Application For Detection Of Duck And Chicken Reovirus Infections. Chen, XM, Li, TT, Chen, XD, Li, CX, Lin, WW, Liu, HY, Song, SP, Bai, XF, Zhang, Y. 2019

作者其他论文 更多>>