数字农科院2.0

Ras-Like Family Small Gtpases Genes I.n Nilaparvata Lugens: Identification, P hylogenetic Analysis, Gene Expression And Function In Nymphal Development

文献类型： 外文期刊

作者： Wang, WX; Li, KL; Wan, PJ; Lai, FX; Fu, Q; Zhu, TH

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期刊名称： PLOS ONE

ISSN： 1932-6203

年卷期： 2017 年 12 卷 2 期

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收录情况： JCR（2021版）

摘要： Twenty-nine cDNAs encoding Ras-like family small GTPases (RSGs) were cloned and sequenced from Nilaparvata lugens. Twenty-eight proteins are described here: 3 from Rho, 2 from Ras, 9 from Arf and 14 from Rabs. These RSGs from N.lugens have five conserved G-loop motifs and displayed a higher degree of sequence conservation with orthologues from insects. RT-qPCR analysis revealed NIRSGs expressed at all life stages and the highest expression was observed in hemolymph, gut or wing for most of NIRSGs. RNAi demonstrated that eighteen NIRSGs play a crucial role in nymphal development. Nymphs with silenced NIRSGs failed to molt, eclosion or development arrest. The qRT-PCR analysis verified the correlation between mortality and the down-regulation of the target genes. The expression level of nuclear receptors, Kr-h1, Hr3, FTZ-F1 and E93 involved in 20E and JH signal pathway was impacted in nymphs with silenced twelve NIRSGs individually. The expression of two halloween genes, Cyp314a1 and Cyp315a1 involved in ecdysone synthesis, decreased in nymphs with silenced NISar1 or NIArf1. Cyp307a1 increased in nymphs with silenced NIArf6. In N.lugens with silenced NISR beta, NISar1 and NIRab2 at 9th day individually, 0.0% eclosion rate and almost 100.0% mortality was demonstrated. Further analysis showed NISR beta could be served as a candidate target for dsRNA-based pesticides for N.lugens control.

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