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Bta-Mir-23A Involves In Adipogenesis Of P.rogenitor Cells Derived From F etal Bovine Skeletal Muscle

文献类型： 外文期刊

作者： Guan, L; Hu, X; Liu, L; Xing, YS; Zhou, ZK; Liang, XW; Yang, QY; Jin, SY; Bao, JS; Gao, HJ; Du, M; Li, JY; Zhang, LP

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期刊名称： SCIENTIFIC REPORTS

ISSN： 2045-2322

年卷期： 2017 年 7 卷

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收录情况： JCR（2021版）

摘要： Intramuscular fat deposition or marbling is essential for high quality beef. The molecular mechanism of adipogenesis in skeletal muscle remains largely unknown. In this study, we isolated Platelet-derived growth factor receptor alpha (PDGFR alpha) positive progenitor cells from fetal bovine skeletal muscle and induced into adipocytes. Using miRNAome sequencing, we revealed that bta-miR-23a was an adipogenic miRNA mediating bovine adipogenesis in skeletal muscle. The expression of bta-miR-23a was down-regulated during differentiation of PDGFR alpha+ progenitor cells. Forced expression of bta-miR-23a mimics reduced lipid accumulation and inhibited the key adipogenic transcription factor peroxisome proliferative activated receptor gamma (PPAR gamma) and CCAAT/enhancer binding protein alpha (C/EBP alpha). Whereas down-regulation of bta-miR-23a by its inhibitors increased lipid accumulation and expression of C/EBP alpha, PPAR gamma and fatty acid-binding protein 4 (FABP4). Target prediction analysis revealed that ZNF423 was a potential target of bta-miR-23a. Dual-luciferase reporter assay revealed that bta-miR-23a directly targeted the 3'-UTR of ZNF423. Together, our data showed that bta-miR-23a orchestrates early intramuscular adipogeneic commitment as an anti-adipogenic regulator which acts by targeting ZNF423.

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