数字农科院2.0

Occurrence Of Soil Insect Pests: I.nsight From Classical Identification S upplemented With Dna Barcoding

文献类型： 外文期刊

作者： Nyamwasa, I.;Waweru, B.;Zhang, S.;Yin, J.;Li, H.;Li, K.;Li, H.;Kajuga, J.;Hategekimana, A.

作者机构：

关键词： Soil insect; scarab beetles; white grub; cytochrome c; oxidase I; DNA barcoding

期刊名称： INTERNATIONAL JOURNAL OF PEST MANAGEMENT

ISSN： 0967-0874

年卷期： 2017 年 63 卷 1.0 期

页码：

收录情况： JCR（2021版）

摘要： White grubs are key vegetables soil pests in Rwanda. However, aforementioned insect pests are unknown. We monitored adults with light traps and excavated larvae during cropping seasons of 2014 and 2015. Totally, 42 species were collected. The peak flights of key insect pests occurred from August to October and mid-March. The cytochrome C oxidase I (COI) gene was amplified with polymerase chain reaction (PCR) and used to compare larval DNA against DNA from adults by using MEGA 6, GMYC and ABGD software. The results obtained validate the use of COI gene. The identification results will help to predict critical time for management practices.

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