数字农科院2.0

De Novo Dna Methylation During M.onkey Pre-Implantation Embryogenesis

文献类型： 外文期刊

作者： Sun, Nianqin;Sun, Nianqin;Sun, Yi Eve;Si, Wei;Si, Chenyang;Si, Chenyang;Si, Wei;Wang, Hong;Wang, Hong;Yu, Juehua;Tan, Tao;Sun, Yi Eve;Sun, Yi Eve;Tan, Tao;Ji, Weizhi;Kang, Yu;Kang, Yu;Ji, Weizhi;Chen, Yongchang;Chen, Yongchang;Gao, Fei;Luo, Yuping;Niu, Yuyu;Niu, Yuyu;Lu, Hanlin;Li, Chang;Li, Chang;Li, Siguang

作者机构：

关键词： rhesus monkey; pre-implantation embryogenesis; de novo DNA methylation

期刊名称： CELL RESEARCH

ISSN： 1001-0602

年卷期： 2017 年 27 卷 4.0 期

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收录情况： JCR（2021版） ; CSCD（2020-2021年度） ; 科技核心（2020版）

摘要： Critical epigenetic regulation of primate embryogenesis entails DNA methylome changes. Here we report genome-wide composition, patterning, and stage-specific dynamics of DNA methylation in pre-implantation rhesus monkey embryos as well as male and female gametes studied using an optimized tagmentation-based whole-genome bisulfite sequencing method. We show that upon fertilization, both paternal and maternal genomes undergo active DNA demethylation, and genome-wide de novo DNA methylation is also initiated in the same period. By the 8-cell stage, remethylation becomes more pronounced than demethylation, resulting in an increase in global DNA methylation. Promoters of genes associated with oxidative phosphorylation are preferentially remethylated at the 8-cell stage, suggesting that this mode of energy metabolism may not be favored. Unlike in rodents, X chromosome inactivation is not observed during monkey pre-implantation development. Our study provides the first comprehensive illustration of the 'wax and wane' phases of DNA methylation dynamics. Most importantly, our DNA methyltransferase loss-of-function analysis indicates that DNA methylation influences early monkey embryogenesis.

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