数字农科院2.0

Molecular Characterization And Expression Profile O.f Nanos In Schistosoma J aponicum And Its Influence On The Expression Several Mammalian Stem Cell Factors

文献类型： 外文期刊

作者： Giri, BR; Du, XL; Xia, TQ; Chen, YJ; Li, H; Cheng, GF

作者机构：

关键词： Nanos; Stemcells; Expression; Function; Schistosoma japonicum

期刊名称： PARASITOLOGY RESEARCH

ISSN： 0932-0113

年卷期： 2017 年 116 卷 7 期

页码：

收录情况： JCR（2021版）

摘要： Pluripotent stem cells, called neoblasts, are well known for the regenerative capability and developmental plasticity in flatworms. Impressive advancement has been made in free-living flatworms, while in case of its parasitic counterpart, neoblast-like stem cells have attracted recent attention for its self-renewal and differentiation capacity. Nanos is a key conserved post-transcriptional regulator critical for the formation, development, and/or maintenance of the pluripotent germ line stem cell systems in many metazoans including schistosomes. In the present study, we report the molecular cloning and expression of nanos orthologous genes nanos in Schistosoma japonicum (Sjnanos). The cDNA of Sjnanos is 826 bp long, containing an open reading frame (ORF) for 223 amino acid long protein. qRT-PCR analysis shown that Sjnanos was differently expressed in several stages of schistosomes with relatively high level in schistosomula. Additionally, Sjnanos was expressed highly in adult females compared to adult males. Transfection of recombinant plasmid for expressing Sjnanos resulted in significant proliferation and increased expression of several stem cell factors in mammalian cells. Overall, our preliminary study provides the molecular basis to further functionally characterize Sjnanos in S. japonicum.

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