数字农科院2.0

Constitutive Expression Of Feedback-Insensitive Cystathionine G.amma-Synthase Increases Methionine Levels I n Soybean Leaves And Seeds

文献类型： 外文期刊

作者： Hou Wen-sheng;Hacham, Yael;Han Tian-fu;Song Shi-kui;Hou Wen-sheng;Matityahu, Ifat;Hacham, Yael;Wu Cun-xiang;Sun Shi;Yu Yang;Amir, Rachel;Amir, Rachel

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关键词： soybean (Glycine max (L.) Merr.); constitutive expression; cystathionine gamma-synthase (CGS); methionine content; storage protein

期刊名称： JOURNAL OF INTEGRATIVE AGRICULTURE

ISSN： 2095-3119

年卷期： 2018 年 17 卷 1 期

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摘要： Soybean (Glycine max (L.) Merr.) is a major crop that provides plant-origin protein and oil for humans and livestock. Although the soybean vegetative tissues and seeds provide a major source of high-quality protein, they suffer from low concentration of an essential sulfur-containing amino acid, methionine, which significantly limits their nutritional quality. The level of methionine is mainly controlled by the first unique enzyme of methionine synthesis, cystathione gamma-synthase (CGS). Aiming to elevate methionine level in vegetative tissues and seeds, we constitutively over-expressed a feedback-insensitive Arabidopsis CGS (AtD-CGS) in soybean cultivars, Zigongdongdou (ZD) and Jilinxiaoli 1 (JX). The levels of soluble methionine increased remarkably in leaves of transgenic soybeans compared to wild-type plants (6.6-and 7.3-fold in two transgenic ZD lines, and 3.7-fold in one transgenic JX line). Furthermore, the total methionine contents were significantly increased in seeds of the transgenic ZD lines (1.5-to 4.8-fold increase) and the transgenic JX lines (1.3-to 2.3-fold increase) than in the wild type. The protein contents of the transgenic soybean seeds were significantly elevated compared to the wild type, suggesting that the scarcity of methionine in soybeans may limit protein accumulation in soybean seeds. The increased protein content did not alter the profile of major storage proteins in the seeds. Generally, this study provides a promising strategy to increase the levels of methionine and protein in soybean through the breeding programs.

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