数字农科院2.0

Microrna-95 Promotes Myogenic Differentiation By D.own-Regulation Of Aminoacyl-Trna Synthase C omplex-Interacting Multifunctional Protein 2

文献类型： 外文期刊

作者： Li, B; Xie, SS; Cai, CB; Qian, LL; Jiang, SW; Ma, DZ; Xiao, GJ; Gao, T; Yang, JZ; Cui, WT

作者机构：

关键词： myostatin; miR-95; AIMP2; C2C12; differentiation

期刊名称： ONCOTARGET

ISSN： 1949-2553

年卷期： 2017 年 8 卷 67 期

页码：

收录情况： SCI

摘要： MicroRNA-95 (miR-95) is well known for its ability to promote the proliferation of a variety of cancer cells, but its function in skeletal muscle development has not been reported so far. Our laboratory has recently generated genetically engineered Meishan pigs containing a loss-of-function myostatin (MSTN) mutant (MSTN-/-). These MSTN-/- pigs grow and develop normally but show clear double muscle phenotype as observed in Belgian cattle. We observed that the expression of miR-95 was upregulated in the longissimus dorsi from MSTN-/- Meishan pigs at day 65 during embryo development. In this study, we investigated the role of miR-95 in the myogenic differentiation using a murine myoblast cell line C2C12. Our results revealed that miR-95 may play a very important role in regulating the expression of myogenic differentiation marker genes myosin heavy chain (MHC) and myogenin. By use of bioinformatical analysis and luciferase reporter gene assay, aminoacyl-tRNA synthase complex-interacting multifunctional protein 2 (AIMP2) gene was identified as a miR-95 target gene involved in myogenic differentiation. Our results indicated that higher miR-95 expression level leads to lower level of AIMP2 protein expression. When the endogenous expression of AIMP2 is inhibited by siRNA, the expression levels of myogenic differentiation marker genes MHC and myogenin increased, implying that AIMP2 negatively regulates myogenic differentiation. Taken together, it is likely that miR-95 promotes myogenic differentiation in C2C12 myoblasts and may play a positive functional role in skeletal muscle development by down regulating the expression of AIMP2 at protein level.

分类号：

  • 相关文献

[1]东北马鹿Myostatin基因编码序列的克隆及序列分析. 巴恒星,邢秀梅,李春义,杨福合. 2010

[2]北京鸭肌肉生长抑制素基因多态与屠体性状关系分析. 卢俊清,黄苇,赵楠,王文武,侯水生. 2008

[3]绵羊Myostatin基因真核表达载体的构建及在其原代成纤维细胞中的瞬时表达. 陆健,宋正海,吕延飞,赵福平,张莉. 2012

[4]Myostatin downregulates the expression of basic fibroblast growth factor gene in HeLa cells. Shang, J. H.,Liu, H. Z.,Chen, S. H.,Luo, P.. 2012

[5]Stimulation with monochromatic green light during incubation alters satellite cell mitotic activity and gene expression in relation to embryonic and posthatch muscle growth of broiler chickens. Zhang, L.,Zhang, H. J.,Wang, J.,Wu, S. G.,Qiao, X.,Yue, H. Y.,Qi, G. H.,Zhang, L.,Yao, J. H.. 2014

[6]Functional verification of a porcine myostatin propeptide mutant. Ma, Dezun,Jiang, Shengwang,Gao, Pengfei,Wang, Qingqing,Xiao, Gaojun,Cui, Wentao,Qian, Lili,Cai, Chunbo,Yang, Jinzeng.

[7]Myostatin knockout using zinc-finger nucleases promotes proliferation of ovine primary satellite cells in vitro. Salabi, Fatemeh,Chen, Qing,Nimal, Jonathan,Cao, Wen. G.,Nazari, Mahmood,Tong, Jianming.

[8]Transcript characteristic of myostatin in sheep fibroblasts. Lu, Jian,Ding, Jiatong,Li, Bichun,Lu, Jian,Zhang, Xiaoning,Zhao, Fuping,Zhou, Xinlei,Zhang, Li,Wei, Caihong,Du, Lixin,Ren, Hangxing,Sheng, Xihui,Li, Shangang.

[9]The effect of myostatin silencing by lentiviral-mediated RNA interference on goat fetal fibroblasts. Lu, Jian,Wei, Caihong,Zhang, Xiaoning,Xu, Lingyang,Zhang, Shifang,Liu, Jiasen,Cao, Jiaxue,Zhao, Fuping,Zhang, Li,Du, Lixin,Lu, Jian,Lu, Jian,Li, Bichun,Liu, Jiasen,Cao, Jiaxue.

[10]Selection of an effective small interference RNA to silence myostatin gene expression in sheep fibroblast cells. Lu, Jian,Ding, Jiatong,Li, Bichun,Lu, Jian,Xu, Lingyang,Lu, Guobin,Zhao, Fuping,Wei, Caihong,Zhang, Li,Du, Lixin,Sun, Dan.

[11]Effects of Different Generations and Sex on Physiological, Biochemical, and Growth Parameters of Crossbred Beef Cattle by Myostatin Gene-Edited Luxi Bulls and Simmental Cows. Chao Hai,Chunling Bai,Lei Yang,Zhuying Wei,Hong Wang,Haoran Ma,Haibing Ma,Yuefang Zhao,Guanghua Su,Guangpeng Li. 2023

[12]Enhanced Skeletal Muscle Growth In Myostatin-Deficient Transgenic Pigs Had Improved Glucose Uptake In Stretozotocin-Induced Diabetes. Li, B, Cui, WT, Yang, JZ. 2020

[13]An Integrated Analysis Of Mrna A.nd Mirna In Skeletal M uscle From Myostatin-Edited Meishan Pigs. Xie, SS, Li, X, Qian, LL, Cai, CB, Xiao, GJ, Jiang, SW, Li, B, Gao, T, Cui, WT. 2019

[14]Myostatin Alteration in Pigs Enhances the Deposition of Long‐Chain Unsaturated Fatty Acids in Subcutaneous Fat. Yangli Pei,Yuxin Song,Zheng Feng,Hua Li,Yulian Mu,Saif Ur Rehman,Qingyou Liu,Kui Li. 2022

[15]Viscera Characteristics of MSTN-Edited Heterozygous Pigs. Yangli Pei,Ziyao Fan,Yuxin Song,Chujie Chen,Yulian Mu,Bugao Li,Zheng Feng,Hua Li,Kui Li. 2022

[16]MiR-208b Regulates the Conversion of Skeletal Muscle Fiber Types by Inhibiting Mettl8 Expression. Xiang Li,Hanfang Bi,Shanshan Xie,Wentao Cui. 2022

[17]Optimization of mating strategy with genomic information for genetic advantage and defect based on linear programming in Huaxi cattle. Yuanqing Wang,Bo Zhu,Yibing Yue,Jiaxin Zhang,Lupei Zhang,Lingyang Xu,Yan Chen,Zezhao Wang,Huijiang Gao,Xue Gao,Junya Li. 2025

[18]Population Genetic Dynamics Of Himalayan-Hengduan T.ree Peonies, Paeonia Subsect. D elavayanae. Zhang, JM, Lopez-Pujol, J, Gong, X, Wang, HF, Vilatersana, R, Zhou, SL. 2018

[19]Microrna-34C Acts As A Bidirectional S.witch In The Maturation O f Insulin-Producing Cells Derived From Mesenchymal Stem Cells. Bai, CY, Gao, YH, Zhang, XY, Yang, WC, Guan, WJ. 2017

[20]Biological Characterization And Pluripotent Identification O.f Ovine Amniotic Fluid S tem Cells. Pei, WH, Lu, TF, Wang, KF, Ji, M, Zhang, S, Chen, FH, Li, L, Li, XC, Guan, WJ. 2018

作者其他论文 更多>>