数字农科院2.0

Transgenic Cotton Plants Expressing The H.ahr3 Gene Conferred Enhanced R esistance To Helicoverpa Armigera And Improved Cotton Yield

文献类型： 外文期刊

作者： Li, Shupeng;Wang, Zhenzhen;Zeng, Hongmei;Zhang, Zhigang;Xiong, Yehui;Qiu, Dewen;Lv, Shun;He, Yunxin;Han, Qiang

作者机构：

关键词： RNA interference; plant mediated-RNAi; Helicoverpa armigera; HaHR3; transgenic cotton plants

期刊名称： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

ISSN： 1422-0067

年卷期： 2017 年 18 卷 9.0 期

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收录情况： SCI

摘要： RNA interference (RNAi) has been developed as an efficient technology. RNAi insect-resistant transgenic plants expressing double-stranded RNA (dsRNA) that is ingested into insects to silence target genes can affect the viability of these pests or even lead to their death. HaHR3, a molt-regulating transcription factor gene, was previously selected as a target expressed in bacteria and tobacco plants to control Helicoverpa armigera by RNAi technology. In this work, we selected the dsRNA-HaHR3 fragment to silence HaHR3 in cotton bollworm for plant mediated-RNAi research. A total of 19 transgenic cotton lines expressing HaHR3 were successfully cultivated, and seven generated lines were used to perform feeding bioassays. Transgenic cotton plants expressing dsHaHR3 were shown to induce high larval mortality and deformities of pupation and adult eclosion when used to feed the newly hatched larvae, and 3rd and 5th instar larvae of H. armigera. Moreover, HaHR3 transgenic cotton also demonstrated an improved cotton yield when compared with controls.

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