数字农科院2.0

Identification Of An Operon Involved I.n Fluoride Resistance In E nterobacter Cloacae Frm

文献类型： 外文期刊

作者： Liu, XQ; Tian, J; Liu, LH; Zhu, T; Yu, XX; Chu, XY; Yao, B; Wu, NF; Fan, YL

作者机构：

期刊名称： SCIENTIFIC REPORTS

ISSN： 2045-2322

年卷期： 2017 年 7 卷

页码：

收录情况： JCR（2021版）

摘要： Fluorine is ubiquitous and the most active non-metal element in nature. While many microorganisms have developed fluoride resistance as a result of the widespread and prolonged application of oral hygiene products, the mechanisms used by these organisms to overcome fluoride toxicity are incompletely understood. In this study, a fluoride-resistant strain, Enterobacter cloacae FRM, was identified which could grow well at a fluoride concentration of 4,000 mg/L. According to comparative genomics, transcriptome under fluoride stress, and sequence analyses of two fluoride-resistant fosmid clones, the genomic island GI3 was found to be important for fluoride resistance. The result of quantitative RT-PCR indicated that six genes on GI3, ppaC, uspA, eno, gpmA, crcB, and orf5249, which encode a fluoride transporter, fluoride-inhibited enzymes, and a universal stress protein, reside in an operon and are transcribed into two mRNAs activated by fluoride with a fluoride riboswitch. The results of knockout and complementation experiments indicated that these genes work together to provide high fluoride resistance to E. cloacae FRM. This study clarified the resistance mechanism of this high fluoride-resistant organism and has expanded our understanding of the biological effects of fluoride.

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