数字农科院2.0

Optimization Of Solid State Fermentation P.rocess For Protein Enrichment O f Potato Starch Residue With Mixed Strains

文献类型： 外文期刊

作者： Lei, Heng;Xu, Chuncheng;Hao, Wei;Wang, Xiaoli;Ning, Tingting;Wang, Huili

作者机构：

关键词： optimization; potato starch residue; protein nitrogen; response surface methodology

期刊名称： ENVIRONMENTAL ENGINEERING AND MANAGEMENT JOURNAL

ISSN： 1582-9596

年卷期： 2017 年 16 卷 7 期

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摘要： This study was to optimize the fermentation process for protein enrichment of potato starch residue (PSR) by solid state fermentation with mixed strains. Three single-factor experiments were conducted to optimize the fermentation conditions, and a Plackett-Burman design followed by a central composite design of response surface methodology was investigated to optimize the medium constituents. The optimal fermentation conditions were fermentation temperature 28 degrees C, fermentation period 72 h and solids content was 40%. A combination medium of 19.75 g potato starch residue, 6.38 g wheat bran, 2.86 g corn meal, 2.62 g urea, 0.15 g K2HPO4, 0.15 g MgSO4 center dot 7H(2)O, 0.075 g MnSO4 center dot H2O and 0.015 g Fe-2(SO4)(3) was optimum for maximum protein nitrogen content (3.75%) of fermented PSR, which was 3.4 fold over the raw material. The determination coefficient R-2 (0.9889) and the adjusted determination coefficient R-2(0.9790) indicated that the response equation provides a reasonable model for the central composite design experiment, and the lack of fit (P=0.0582) indicated that the model was adequate for prediction within the range of variables employed. Due to the high-yield of protein nitrogen obtained and the low-cost nature of the optimal medium, this study indicates a possibility to establish production of protein feed using PSR as a basic substrate.

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