数字农科院2.0

Dissecting The Role Of Kruppel H.omolog 1 In The M etamorphosis And Female Reproduction Of The Cotton Bollworm, Helicoverpa Armigera

文献类型： 外文期刊

作者： Liu, C.;Liang, Ge.Mei.;Chen, L.;Ma, L.;Zhang, W. -N.;Xiao, Hai. -Jun.;Ma, L.;Zhang, W. -N.

作者机构：

关键词： juvenile hormone; Kruppel homolog 1; metamorphosis; reproduction; RNA interference

期刊名称： INSECT MOLECULAR BIOLOGY

ISSN： 0962-1075

年卷期： 2018 年 27 卷 4.0 期

页码：

收录情况： JCR（2021版）

摘要： In insects, metamorphosis and reproduction are controlled by juvenile hormone (JH) and 20-hydroxyecdysone (20E). Kruppel homolog 1 (Kr-h1), a transcription factor, is regarded as a JH-early inducible gene responsible for the repression of metamorphosis. However, the role of Kr-h1 in reproduction of holometabolic insects is relatively less understood. In this study, we studied the role of Kr-h1 in larvae-pupae transition and female reproduction in the major agricultural pest Helicoverpa armigera. Two HaKr-h1 isoforms (HaKr-h1 and HaKr-h1 beta) were identified, with HaKr-h1 predominant in the cotton bollworm. In larvae, HaKr-h1 was predominately expressed in the epidermis and markedly up-regulated during the moult stage, whereas in adults HaKr-h1 was mainly expressed in females and the highest transcription was detected in the ovaries. Considering the function of hormones in larval metamorphosis, we examined the modulation of gene expression in response to hormones, which showed that HaKr-h1 was significantly induced by both JH analogue (JHA) and 20E. Knockdown of HaKr-h1 in fifth-instar larvae resulted in precocious metamorphosis from larvae to pupae. Moreover, a fluorescence immunoassay coupled with heterologous expression revealed that HaKr-h1 was localized in the nucleus of oocyte membrane. In female adults, depletion of HaKr-h1 severely repressed the transcription of vitellogenin, disrupted oocyte maturation and reduced the number of eggs laid, suggesting that HaKr-h1 is required for vitellogenesis and egg production in H.armigera. The present study provides insight into the roles of HaKr-h1 in JH-mediated reproduction and highlights HaKr-h1 as a target for suppression of lepidopteran pests.

分类号：

  • 相关文献

[1]The Alternative Splicing Of Bdtai A.nd Its Involvement In T he Development Of Bactrocera Dorsalis (Hendel). Yue, Yong,Song, Zhao Hao,Liu, Hong,Li, Hui Min,Dou, Wei,Wang, Jin Jun. 2017

[2]Involvement Of Met And Kr-H1 I.n Jh-Mediated Reproduction Of F emale Bactrocera Dorsalis (Hendel). Yang, Rui-Lin,Chen, Er-Hu,Wang, Wei-Ping,Yue, Yong,Zhou, Qi-Hao,Zhou, Qi-Hao,Yuan, Guo-Rui,Wang, Wei-Ping,Yue, Yong,Wang, Jin-Jun,Wang, Jin-Jun,Dou, Wei,Dou, Wei,Yang, Rui-Lin,Yuan, Guo-Rui,Chen, Er-Hu. 2018

[3]Ocomcsp12, A Chemosensory Protein Expressed Specifically By Ovary, Mediates Reproduction In Ophraella Communa (Coleoptera: Chrysomelidae). Cui, Shaowei,Zhou, Zhongshi,Guo, Jianying,Zhang, Yan,Gao, Xuyuan,Chen, Hongsong,Chen, Hongsong,Chen, Guangmei,Tian, Zhenqi,Gao, Xuyuan,Cui, Shaowei,Tian, Zhenya,Ma, Chao. 2019

[4]Effects of four prey species on the fitness of Amblyseius gramineous (Acari: Phytoseiidae). CHUANTAO HUANG,,HONGNA WANG,,FUJING SHENG,,ENDONG WANG,,BO ZHANG,,XUENONG XU. 2025

[5]Reproduction response of Colletotrichum fungi under the fungicide stress reveals new aspects of chemical control of fungal diseases. Han Y.-C., Zeng X.-G., Guo C., Zhang Q.-H., Chen F.-Y., Ren L., Chen W.-D., Qin L.. 2022

[6]Transient Expression–Mediated Gene Silencing in Plants and Suppression of Gene Silencing with Viral Suppressors. Li F., Wang A.. 2022

[7]Bmsd Gene Regulates The Silkworm W.ing Size By Affecting T he Hippo Pathway. Sun, Xia,Li, Mu:Wang,Qin, Sheng,Hou, Cheng:Xiang,Zhang, Guo:Zheng,Sun, Xia,Li, Mu:Wang,Qin, Sheng,Hou, Cheng:Xiang,Li, Tao,Zhang, Jing,Yin, Jin,Zhang, Guo:Zheng. 2019

[8]Genome-Wide Analysis Of Micrornas In R.elation To Pupariation In O riental Fruit Fly. Dou, Wei,Wang, Jin-Jun,Dou, Wei,Niu, Jin-Zhi,Niu, Jin-Zhi,Dou, Wei,Niu, Jin-Zhi,Chen, Er-Hu,Niu, Jin-Zhi,Chen, Er-Hu,Pan, Deng,Dou, Wei,Chen, Er-Hu,Chen, Er-Hu,Smagghe, Guy,Smagghe, Guy,Smagghe, Guy,Pan, Deng,Smagghe, Guy,Pan, Deng,Pan, Deng,Wang, Jin-Jun,Zhang, Qiang,Zhang, Qiang,Zhang, Qiang,Zhang, Qiang,Wang, Jin-Jun,Wang, Jin-Jun. 2019

[9]Rna-Seq Analysis Of Gene Expression C.hanges During Pupariation In B actrocera Dorsalis (Hendel) (Diptera: Tephritidae). Wei, Dan-Dan,Yu, Shuai-Feng,Yang, Rui-Lin,Yue, Yong,Smagghe, Guy,Wang, Jin-Jun,Smagghe, Guy,Smagghe, Guy,Chen, Er-Hu,Wang, Jin-Jun,Dou, Wei,Wei, Dan-Dan,De Schutter, Kristof,Hou, Qiu-Li,Dou, Wei. 2018

[10]The Roles Of E93 And K.r-H1 In Metamorphosis Of N ilaparvata Lugens. Wan, Pin Jun,Fu, Qiang,Lai, Feng Xiang,Nanda, Satyabrata,Li, Kai Long,Li, Kai Long,Yuan, San Yue,Wang, Wei Xia. 2018

[11]The Physiological Differentiation Along The M.idgut Of Bombyx Mori - Inspirations From Proteomics And Gene Expression Patterns Of The Secreted Proteins In The Ectoperitrophic Space. Yang, Qing,Liu, L.,Yang, Qing,Yang, J.,Qu, M.. 2018

[12]Transcriptional identification of differentially expressed genes during the prepupal–pupal transition in the oriental armyworm, Mythimna separata (Walker) (Lepidoptera: Noctuidae). Peirong Li,Xinru Li,Wei Wang,Xiaoling Tan,Xiaoqi Wang,Xueqing Yang. 2021

[13]Molecular Characterization And Functional Analysis Of The Halloween Genes And Cyp18A1 In Bemisia Tabaci Med. Liu, SN, He, C, Liang, JJ, Su, Q, Hua, DK, Wang, SL, Wu, QJ, Xie, W, Zhang, YJ. 2020

[14]Reproductive Cost Associated With Juvenile Hormone in Bt-Resistant Strains of Helicoverpa armigera (Lepidoptera: Noctuidae). Wang, B. J.,Chen, L.,Khaing, M. M.,Lu, Y. H.,Liang, G. M.,Guo, Y. Y.,Ma, L..

[15]Juvenile hormone membrane signaling phosphorylates USP and thus potentiates 20-hydroxyecdysone action in Drosophila. Yue Gao,Suning Liu,Qiangqiang Jia,Lixian Wu,Dongwei Yuan,Emma Y. Li,Qili Feng,Guirong Wang,Subba R. Palli,Jian Wang,Sheng Li. 2022

[16]MicroRNA let-7-5p targets the juvenile hormone primary response gene Krüppel homolog 1 and regulates reproductive diapause in Galeruca daurica. Tian Feng Duan;Shu Jing Gao,Hai Chao Wang,Ling Li,Yan Yan Li,Yao Tan,Bao Ping Pang. 2022

[17]Biological Characteristics and Energy Metabolism of Migrating Insects. Xiaokang Li,Yan Zhou,Kongming Wu. 2023

[18]Effects of triflumezopyrim on the reproduction of brown planthopper, Nilaparvata lugens. Yao Li,Jianqi Liu,Wenqing Sun,Fang Liu. 2021

[19]The biosynthesis of alarm pheromone in the wheat aphid Rhopalo-siphum padi is regulated by hormones via fatty acid metabolism. Chengxian Sun,Yaoguo Qin,Julian Chen,Zhengxi Li. 2024

[20]Juvenile hormone inhibits lipogenesis of Spodoptera exigua to response to Bacillus thuringiensis GS57 infection. Bo Gao,Yujie Ji,Dan Zhao,Yitong Yan,Lu Zhang,Han Wu,Yifan Xie,Qiuyu Shi,Yao Wang,Wei Guo. 2024

作者其他论文 更多>>