数字农科院2.0

Genetic Diversity Analysis Of Brassica S.pecies Using Pcr-Based Ssr M arkers

文献类型： 外文期刊

作者： Raza, A; Mehmood, SS; Ashraf, F; Khan, RSA

作者机构：

关键词： Alleles; Dendrogram; Gel electrophoresis; Molecular markers; Polymorphic information content

期刊名称： GESUNDE PFLANZEN

ISSN： 0367-4223

年卷期： 2019 年 71 卷 1 期

页码：

收录情况： JCR（2021版）

摘要： Genetic diversity is an important measure for the improvement of many crop species including Brassica. This study evaluated the genetic divergence among six Brassica species using simple sequence repeats (SSR). Ten SSR markers produced overall 21alleles with an average of 2.1alleles per primer. Out of 21, 18alleles showed polymorphism (85.71%) and 3alleles showed monomorphism (14.28%). Asimilarity matrix was constructed using Popgen32 software. Genetic identity ranged from 33.33 to 76.19%. B.nigra and B.campestris showed the highest identity, while the lowest identity was observed between B.campestris and B.oleracea. Sizes of amplified alleles were ranged from 70 to 290bp. Polymorphic information content (PIC) varied from 0.37 to 0.71, with an average of 0.66 per primer. Adendrogram classified the genotypes into two main clusters. Cluster-A is further divided into cluster-C, which consists of B.carinata and B.oleracea. B.napus and B.juncea each form an independent cluster. Cluster-B consists of B.nigra and B.campestris, meaning that these two species are closely related to each other. The results indicated that these species can be isolated from each other at the molecular level by using molecular markers. ZusammenfassungDie genetische Diversitat ist ein wichtiges Ma ss fur die Verbesserung vieler Kulturpflanzen einschlie ss lich Brassica. In der vorliegenden Studie wurde die genetische Divergenz zwischen 6Brassica-Arten unter der Verwendung von Mikrosatelliten (simple sequence repeats, SSR) untersucht. Dabei produzierten 10SSR-Marker insgesamt 21Allele mit einem Durchschnitt von 2,1Allelen pro Primer. Von den 21Allelen zeigten 18Polymorphismus (85,71%), und 3Allele zeigten Monomorphismus (14,28%). Mit der Popgen32-Software wurde eine Ahnlichkeitsmatrix erstellt. Die genetische Identitat lag im Bereich von 33,33-76,19%. Die hochste Identitat wiesen B.nigra und B.campestris auf, wahrend die niedrigste Identitat zwischen B.campestris und B.oleracea beobachtet wurde. Die Gro ss en der amplifizierten Allele lagen im Bereich von 70-290bp. Der polymorphe Informationsgehalt (PIC) variierte von 0,37-0,71, mit einem Durchschnitt von 0,66 pro Primer. Ein Dendrogramm klassifizierte die Genotypen in 2Hauptcluster. Cluster-A wird weiter unterteilt in Cluster-C, der aus B.carinata und B.oleracea besteht. Jeweils einen unabhangigen Cluster bilden B.napus und B.juncea. Cluster-B besteht aus B.nigra und B.campestris, was bedeutet, dass diese beiden Arten eng miteinander verwandt sind. Die Ergebnisse zeigten, dass diese Spezies auf molekularer Ebene unter Verwendung molekularer Marker voneinander isoliert werden konnen.

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