数字农科院2.0

Synergistic Effect Of Acetyl Xylan E.sterase From Talaromyces Leycettanus J cm12802 And Xylanase From Neocallimastix Patriciarum Achieved By Introducing Carbohydrate-Binding Module-1

文献类型： 外文期刊

作者： Zhang, YQ; Yang, H; Yu, XR; Kong, HY; Chen, JM; Luo, HY; Bai, YG; Yao, B

作者机构：

关键词： Acetyl xylan esterase; Xylanase; Synergistic effect; CBM1 domain; Xylooligosaccharides

期刊名称： AMB EXPRESS

ISSN： 2191-0855

年卷期： 2019 年 9 卷

页码：

收录情况： JCR（2021版）

摘要： Wheat bran is an effective raw material for preparation xylooligosaccharides; however, current research mainly focuses on alkali extraction and enzymatic hydrolysis methods. Since ester bonds are destroyed during the alkali extraction process, xylanase and arabinofuranosidase are mainly used to hydrolyze xylooligosaccharides. However, alkali extraction costs are very high, and the method also causes pollution. Therefore, this study focuses on elucidating a method to efficiently and directly degrade destarched wheat bran. First, an acidic acetyl xylan esterase (AXE) containing a carbohydrate-binding module-1 (CBM1) domain was cloned from Talaromyces leycettanus JCM12802 and successfully expressed in Pichia pastoris. Characterization showed that the full-length acetyl xylan esterase AXE+CBM1 was similar toe uncovered AXE with an optimum temperature and pH of 55 degrees C and 6.5, respectively. Testing the acetyl xylan esterase and xylanase derived from Neocallimastix patriciarum in a starch-free wheat bran cooperative experiment revealed that AXE+CBM1 and AXE produced 29% and 16% reducing sugars respectively, compared to when only NPXYN11 was used. In addition, introduced the CBM1 domain into NPXYN11, and the results indicated that the CBM1 domain showed little effect on NPXYN11 properties. Finally, the systematically synergistic effects between acetyl xylan esterase and xylanase with/without the CBM1 domain demonstrated that the combined ratio of AXE+CBM1 coming in first and NPXYN11+CBM1s increased reducing sugars by almost 35% with AXE and NPXYN11. Furthermore, each component's proportion remained the same with respect to xylooligosaccharides, with the largest proportion (86%) containing of 49% xylobiose and 37% xylotriose.

分类号：

  • 相关文献

[1]Utility Of Thermostable Xylanases Of M.ycothermus Thermophilus In Generating P rebiotic Xylooligosaccharides. Ma, R, Bai, YG, Huang, HQ, Luo, HY, Chen, SF, Fan, YL, Cai, L, Yao, B. 2017

[2]Rational Design for Broadened Substrate Specificity and Enhanced Activity of a Novel Acetyl Xylan Esterase from Bacteroides thetaiotaomicron. Luyao Wang,Xue Han,Yulu Wang,Xue Wei,Shujun Liu,Shuli Shao,Shaoqing Yang,Lichao Sun,Fengjiao Xin. 2021

[3]A Novel Endo-Beta-1,4-Xylanase Gh30 From D.ickeya Dadantii Dce-01: Clone, E xpression, Characterization, And Ramie Biological Degumming Function. Wang, RJ, Liu, ZC, Cheng, LF, Duan, SW, Feng, XY, Zheng, K, Cheng, Y, Zeng, J. 2019

[4]The Botrytis Cinerea Xylanase Bcxyl1 M.odulates Plant Immunity. Dong, Yijie,Qiu, Dewen,Yang, Xiufen,Yang, Yuankun. 2018

[5]Insights Into Xylan Degradation And H.aloalkaline Adaptation Through Whole-Genome A nalysis Of Alkalitalea Saponilacus, An Anaerobic Haloalkaliphilic Bacterium Capable Of Secreting Novel Halostable Xylanase. Liao, ZY, Holtzapple, M, Yan, YC, SWang, HS, Li, J, Zhao, BS. 2019

[6]Investigation Of Combined Effects Of Xylanase And Glucose Oxidase In Whole Wheat Buns Making Based On Reconstituted Model Dough System. Yang, M, Yue, Y, Liu, LY, Tong, LT, Wang, LL, Ashraf, J, Li, NN, Zhou, XR, Zhou, SM. 2021

[7]The Gh10 And Gh48 Dual-Functional Catalytic Domains From A Multimodular Glycoside Hydrolase Synergize In Hydrolyzing Both Cellulose And Xylan. Chu, YD, Hao, ZZ, Wang, KK, Tu, T, Huang, HQ, Wang, Y, Bai, YG, Wang, YR, Luo, HY, Yao, B, Su, XY. 2019

[8]Insights Into The Roles Of N.on-Catalytic Residues In The A ctive Site Of A Gh10 Xylanase With Activity On Cellulose. Chu, YD, Tu, T, Penttinen, L, Xue, XL, Wang, XY, Yi, ZL, Gong, L, Rouvinen, J, Luo, HY, Hakulinen, N, Yao, B, Su, XY. 2017

[9]Composition And Foam Properties Of Whole Wheat Dough Liquor As Affected By Xylanase And Glucose Oxidase. Liu, LY, Sun, YL, Yue, Y, Yang, JQ, Chen, LY, Ashraf, J, Wang, LL, Zhou, SM, Tong, LT. 2020

[10]Standardized Ileal Digestible Amino Acid And Metabolizable Energy Content Of Wheat From Different Origins And The Effect Of Exogenous Xylanase On Their Determination In Broilers. Lu, PY, Wang, J, Wu, SG, Gao, J, Dong, Y, Zhang, HJ, Qi, GH. 2020

[11]Flower-Like Direct Z-Scheme Ws2/Bi2O2Co3 Photocatalyst W.ith Enhanced Photocatalytic Activity. Cai, Jiumao,Zhou, Jianguo,Li, Li,Zhao, Fengying,Yan, Yunhui,Zhou, Jianguo. 2019

[12]Multivariate Nanocomposites For Electrochemical Sensing I.n The Application Of F ood. Hu, Xianqiao,Lu, Lin,Zhu, Zhiwei. 2019

[13]Risk Assessment For Pesticide Mixtures O.n Aquatic Ecosystems In C hina: A Proposed Framework. Zhou, Xinxin,Li, Shibin,Chen, Lang,Jiang, Hui,Liu, Xingang,Yuan, Shankui,Zhou, Yanming. 2019

[14]Synergistic Effect Of Bioactive Anticarcinogens F.rom Soybean On Anti-Proliferative A ctivity In Mda-Mb-231 And Mcf-7 Human Breast Cancer Cells In Vitro. Zhu, YY, Yao, Y, Shi, ZX, Everaert, N, Ren, GX. 2018

[15]Graphene Oxide As A Pesticide D.elivery Vector For Enhancing A caricidal Activity Against Spider Mites. Wang, Xiuping,Xie, Haicui,He, Kanglai,Wang, Zhengying,Wang, Xiuping. 2019

[16]Treatment Of Landfill Leachate Reverse O.smosis Concentrate From By C atalytic Ozonation With Gamma-Al2O3. He, YL, Zhang, H, Li, JJ, Zhang, YH, Lai, B, Pan, ZC. 2018

[17]“Three-in-one” Zr-MOF Multifunctional Carrier-mediated Fluorescent and Colorimetric Dual-signal Readout Biosensing Platform to Enhance Analytical Performance. Liangqiong Ren, Feng Hong, Lingwen Zeng, and Yiping Chen. 2022

[18]Dual-Functionalized Pesticide Nanocapsule Delivery System With Improved Spreading Behavior And Enhanced Bioactivity. Cui, JX, Sun, CJ, Wang, AQ, Wang, Y, Zhu, HX, Shen, Y, Li, NJ, Zhao, X, Cui, B, Wang, C, Gao, F, Zeng, ZH, Cui, HX. 2020

[19]A Swollenin From Talaromyces leycettanus JCM12802 Enhances Cellulase Hydrolysis Toward Various Substrates. Zhang Honghai,Wang Yuan,Brunecky Roman,Yao Bin,Xie Xiangming,Zheng Fei,Luo Huiying. 2021

[20]Transcriptomic And Proteomic Profiling Reveals The Intestinal Immunotoxicity Induced By Aflatoxin M1 And Ochratoxin A. Gao, YN, Ye, QY, Bao, XY, Huang, X, Wang, JQ, Zheng, N. 2020

作者其他论文 更多>>