数字农科院2.0

Ghklcr1, A Kinesin Light Chain-Related G.ene, Induces Drought-Stress Sensitivity I n Arabidopsis

文献类型： 外文期刊

作者： Lu, Lili;Yu, Daoqian;Yu, Daoqian;Zheng, Lei;Yang, Zuoren;Wu, Huanhuan;Wang, Lingling;Qanmber, Ghulam;Liu, Zhao;Liu, Xiaodong;Li, Jie;Li, Jie;Li, Fuguang;Chen, Quanjia

作者机构：

关键词： GhKLCR1; kinesin; Arabidopsis; mannitol; seed germination; drought sensitivity; GUS; cis-elements

期刊名称： SCIENCE CHINA-LIFE SCIENCES

ISSN： 1674-7305

年卷期： 2019 年 62 卷 1 期

页码：

摘要： Drought stress results in significant losses in agricultural production, and especially that of cotton. The molecular mechanisms that coordinate drought tolerance remain elusive in cotton. Here, we isolated a drought-response gene GhKLCR1, which is a close homolog of AtKLCR1, which encodes a kinesin light chain-related protein enriched with a tetratrico peptide-repeat region. A subcellular localization assay showed that GhKLCR1 is associated with the cell membrane. A tissue-specific expression profile analysis demonstrated that GhKLCR1 is a cotton root-specific gene. Further abiotic and hormonal stress treatments showed that GhKLCR1 was upregulated during abiotic stresses, especially after polyethylene glycol treatments. In addition, the glucuronidase (GUS) staining activity increased as the increment of mannitol concentration in transgenic Arabidopsis plants harboring the fusion construct P-GhKLCR1::GUS. The root lengths of 35S::GhKLCR1 lines were significantly reduced compared with that of wild type. Additionally, seed germination was strongly inhibited in 35S::GhKLCR1 lines after 300-mmol L-1 mannitol treatments as compared with Columbia-0, indicating the sensitivity of GhKLCR1 to drought. These findings provide a better understanding of the structural, physiological and functional mechanisms of kinesin light chain-related proteins.

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