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Cloning, Expression, And Bioinformatics Analysis O.f Heavy Metal Resistance G ene Afe_1862 From Acidithiobacillus Ferrooxidans L1 In Escherichia Coli

文献类型： 外文期刊

作者： Leng, Feifan;Leng, Feifan;Li, Yuanli;Luo, Wen;Jing, Yanjun;Yang, Mingjun;Wang, Yonggang;Wei, Qingwei;Wang, Xiaoli

作者机构：

关键词： Acidithiobacillus ferrooxidans; Metal resistance; Expression; Bioinformatics; afe_1862

期刊名称： BIOLOGICAL TRACE ELEMENT RESEARCH

ISSN： 0163-4984

年卷期： 2019 年 189 卷 1 期

页码：

摘要： Molecular studies of copper and cadmium resistances in acidophilic bacteria are significant in biomining. In this study, afe_1862, which encodes a heavy metal-binding protein in Acidithiobacillus ferrooxidans L1, was amplified using PCR, cloned into the pET32a plasmid, and sequenced. Following SDS-PAGE analysis, optimization of the expression conditions and heterologous overexpression of afe_1862 in Escherichia coli BL21 in the presence of Cu2+ and Cd2+ were studied as well. The results indicated that AFE_1862 has higher resistance to Cu2+ than Cd2+. Bioinformatics analysis illustrated that AFE_1862 has a conserved HMA domain containing heavy metal-binding sites, which may play a role in transporting or detoxifying heavy metals.

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