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Complete Nucleotide Sequence Of A N.ovel Mycovirus From Trichoderma H arzianum In China

文献类型: 外文期刊

作者: Zhang, Jiantai;Wu, Beilei;Redda, Estifanos Tsegaye;Mei, Jie;Liu, Chenchen;Li, Mei;Jiang, Xiliang;Elena, Santiago F.;Elena, Santiago F.

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期刊名称: ARCHIVES OF VIROLOGY

ISSN: 0304-8608

年卷期: 2019 年 164 卷 4 期

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摘要: A new mycovirus was identified in Trichoderma harzianum strain 137 isolated in Xinjiang province, China. The whole genome sequence of the mycovirus was determined by metagenomic sequencing, RT-PCR, and RACE cloning. The mycovirus contained two genomic segments. The first segment was 2088bp long and contained a single ORF (ORF1) encoding the RNA-dependent RNA polymerase (RdRP) (72.26kDa). The second segment was 1634bp long and also contained a single ORF (ORF2) encoding a hypothetical protein of 37.472kDa. We named this novel mycovirus Trichoderma harzianum bipartite mycovirus 1 (ThBMV1). Phylogenetic analysis showed that ThBMV1 clusters with other unclassified dsRNA mycoviruses.

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