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The Role Of Tyramine Beta-Hydroxylase I.n Density Dependent Immunityof O riental Armyworm (Mythmina Separata) Larva

文献类型： 外文期刊

作者： Hailong Kong; Chuanlei Dong; Wanghui Jing; Zhen Tian; Minyuan Zheng;Cheng Wang; Qiuli Hou; Yunxia Cheng; Lei Zhang; Xingfu Jiang; Lizhi Luo;Shude Zhu

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关键词： tyramine beta-hydroxylase; Mythimna separata; octopamine; larval density; prophylactic immunity

期刊名称： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

ISSN： 1422-0067

年卷期： 2019 年 20 卷 7.0 期

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收录情况： SCI

摘要： High population density alters insect prophylactic immunity, with density-dependent prophylaxis (DDP) being reported in many polyphonic insects. However, the molecular mechanism for DDP remains unclear. In current study, the role of tyramine beta-hydroxylase (T beta h) in the immune response of M. separata larvae that were subject to different rearing densities conditions was investigated. The tyramine beta-hydroxylase activity of larvae from high density treatments (10 and 30 larvae per jar) was significantly higher than that of the larvae from low density treatments (one, two, and five larvae/jar). A tyramine beta-hydroxylase (designated MsT beta h) containing a 1779 bp open reading frame was identified. Multiple sequence alignment and phylogenetic analysis indicated that MsT beta h was orthologous to the T beta h that was found in other lepidopterans. Elevated MsT beta h expression was observed in larvae under high density (10 larvae per jar). Silencing MsT beta h expression by the injection of dsRNA in larvae from the high density treatment produced a 25.1% reduction in octopamine levels, while at the same time, there was a significant decrease in phenoloxidase (PO) and lysozyme activity, total haemocyte counts, and survival against Beauveria infection 56.6%, 88.5%, 82.0%, and 55.8%, respectively, when compared to control larvae. Our findings provide the first insights into how MsT beta h mediates the octopamine level, which in turn modulates the immune response of larvae under different population densities.

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