数字农科院2.0

Characterization Of Subtilin L-Q11, A N.ovel Class I Bacteriocin S ynthesized By Bacillus Subtilis L-Q11 Isolated From Orchard Soil

文献类型： 外文期刊

作者： Qin, YX; Wang, Y; He, YH; Zhang, Y; She, QX; Chai, YR; Lp, PL; Shang, QM

作者机构：

关键词： Subtilin L-Q11; Bacillus subtilis; bacteriocin; antibacterial activity; antibacterial mechanism

期刊名称： FRONTIERS IN MICROBIOLOGY

ISSN： 1664-302X

年卷期： 2019 年 10 卷

页码：

收录情况： JCR（2021版）

摘要： Bacteriocins are peptides or proteins synthesized by bacterial ribosomes that show killing or inhibitory activities against different groups of bacteria. Bacteriocins are considered potential alternatives to traditional antibiotics, preservatives in pharmaceutical and food industries. A strain L-Q11 isolated from orchard soil was phylogenetically characterized as Bacillus subtilis based on 16S rRNA gene sequencing analysis. A novel class I bacteriocin (Subtilin L-Q11), was identified and purified from L-Q11 cell-free supernatant in a four-step procedure, including salt precipitation, cation exchange, gel filtration, and reverse-phase high-performance liquid chromatography (RP-HPLC). The molecular mass (3,552.9 Da) of this novel bacteriocin was determined by Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). The purified Subtilin L-Q11 exhibited optimal features in pH tolerance, thermostability, and sensitivity to proteases. Further, Subtilin L-Q11 showed inhibitory activities against a number of bacteria including some human pathogens and food spoilage bacteria, in particular Staphylococcus aureus. All these important features make this novel bacteriocin a potential candidate for the development of a new antibacterial drug or food preservative in the future.

分类号：

  • 相关文献

[1]Improved Antibacterial Effects Of Alkali-Transformed S.aponin From Quinoa Husks A gainst Halitosis-Related Bacteria. Sun, XY, Yang, XS, Xue, P, Zhang, ZG, Ren, GX. 2019

[2]Biological Characteristics Of A New A.ntibacterial Peptide And Its A ntibacterial Mechanisms Against Gram-Negative Bacteria. Pei, Z.,Ying, X.,Tang, Y.,Kong, L.,Ma, H.,Liu, S.,Zhang, H.,Liu, L.,Wang, K.,Zhang, D.,Gao, Y.. 2018

[3]Synergistic Effects Of The Combined A.pplication Of Bacillus Subtilis H 158 And Strobilurins For Rice Sheath Blight Control. Huang, Shiwen,Hou, Yuxuan,Liu, Lianmeng,Xu, Yihua,Sun, Lei,Li, Lu,Sun, Lei,Gao, Jian,Liu, Lianmeng,Xu, Yihua,Huang, Shiwen,Wang, Ling,Liang, Mengqi,Li, Lu. 2018

[4]An Enhanced Vector-Free Allele Exchange (.Vfae) Mutagenesis Protocol For G enome Editing In A Wide Range Of Bacterial Species. Yan, Yongliang,Shang, Liguo,Yang, Zhimin,Zhang, Chen,Zhan, Yuhua,Gomaa, Ahmed E.,Deng, Zhiping,Jiang, Shijie,Lu, Wei,Lin, Min. 2017

[5]Effects Of Long-Term Bacillus Subtilis C.gmcc 1.921 Supplementation On P erformance, Egg Quality, And Fecal And Cecal Microbiota Of Laying Hens. Guo, JR, Dong, XF, Liu, S, Tong, JM. 2017

[6]Isolation And Identification Of A B.acillus Subtilis Hz-72 Exhibiting B iocontrol Activity Against Flax Seedling Blight. Tan, TM, Zhu, JX, Shen, AR, Li, JL, Yu, YT, Zhang, MJ, Zhao, MR, Li, ZM, Chen, J, Gao, CS, Cheng, Y, Guo, LT, Yan, L, Sun, XP, Zeng, LB, Yan, Z. 2019

[7]Transcriptional Profile Of Gene Clusters I.nvolved In The Methylerythritol P hosphate Pathway In Bacillus Subtilis 916. Xiao Ya-jing,Xiao Ya-jing,Peng Qi,Gao Tan-tan,Zhang Jie,Sun Dong-mei,Song Fu-ping. 2019

[8]Seed Treatment Containing Bacillus Subtilis B.y-2 In Combination With O ther Bacillus Isolates For Control Of Sclerotinia Sclerotiorum On Oilseed Rape. Hu, XJ, Roberts, DP, Xie, LH, Qin, L, Li, YS, Liao, XS, Han, PP, Yu, CB, Liao, X. 2019

[9]Engineering Of A Bacillus Subtilis S.train Deficient In Cellulase: A pplication In Degumming Of Ramie. Yang, Q, Duan, SW, Cheng, LF, Feng, XY, Zheng, K, Xie, CL, Liu, ZY, Pen, YD. 2019

[10]Extracellular Production Of Active-Form Streptomyces Mobaraensis Transglutaminase In Bacillus Subtilis. Fu, LH, Wang, Y, Ju, JS, Cheng, L, Xu, YQ, Yu, B, Wang, LM. 2020

[11]In Vitro Efficacy Of Microbial A.ntagonists, Botanical Extracts And S ynthetic Chemicals Against Mango Quick Wilt Pathogen Ceratocystis Manginecans. Ahmad, T, Moosa, A, Rehman, A. 2019

[12]Potent Fungal Chitinase for the Bioconversion of Mycelial Waste. Liu, Tian,Liu, Tian,Yang, Qing,Yang, Qing,Han, Hongyu,Zhou, Yong,Han, Hongyu,Wang, Di,Guo, Xiaoguang,Fukamizo, Tamo,Yang, Qing. 2020

[13]Cell Membrane-Interrupting Antimicrobial Peptides From Isatis Indigotica Fortune Isolated By A Bacillus Subtilis Expression System. 刘允军,,王国英. 2020

[14]Improving The Production Of Ahl Lactonase Aiio-Aio6 Fromochrobactrumsp. M231 In Intracellular Protease-Deficientbacillus Subtilis. Xia, R, Yang, YL, Pan, XL, Gao, CC, Yao, YY, Liu, XW, Teame, T, Zhang, FL, Hu, J, Ran, C, Zhang, Z, Liu-Clarke, JH, Zhou, ZG. 2020

[15]Synthesis And Biological Activity Evaluation O.f Novel Heterocyclic Pleuromutilin D erivatives. Qin, Wenwen,Shang, Ruofeng,Yi, Yunpeng,Liu, Yu,Dong, Pengcheng,Fu, Yunxing,Liang, Jiangping. 2017

[16]Synthesis And Antibacterial Activities Of N.ovel Pleuromutilin Derivatives With A Substituted Pyrimidine Moiety. Liang, Jianping,Huang, Xin,Yi, Yunpeng,Yi, Yunpeng,Shang, Ruofeng,Liu, Yu,Huang, Xin,Xu, Ximing,Xu, Shuijin,Shang, Ruofeng,Liu, Yu,Liang, Jianping. 2017

[17]Cooperative Reinforcement Of Ionic Liquid A.nd Reactive Solvent On E nzymatic Synthesis Of Caffeic Acid Phenethyl Ester As An In Vitro Inhibitor Of Plant Pathogenic Bacteria. Wu, Fu-An,Wu, Fu-An,Xiao, Wei,Xu, Yan,Xu, Yan,Wang, Jun,Sheng, Sheng,Liu, Xi,Sheng, Sheng,Wang, Jun,Wang, Chao. 2017

[18]Antibacterial Activity And Pharmacokinetic Profile O.f A Promising Antibacterial A gent: 14-O-[(4-Amino-6-Hydroxy-Pyrimidine-2-Yl)Thioacetyl] Mutilin. Shang, Ruofeng,Pu, Wanxia,Yi, Yunpeng,Zhang, Chao,Fu, Yunxing,Liang, Jianping. 2018

[19]Recent Advances In Antibacterial And A.ntiendotoxic Peptides Or Proteins F rom Marine Resources. Wang, ZL, Wang, XM, Wang, JH. 2018

[20]Synthesis And Biological Evaluation Of N.ovel Thioether Pleuromutilin Derivatives. Mu, SH, Liu, HX, Zhang, LF, Wang, XY, Xue, FQ, Zhang, Y. 2017

作者其他论文 更多>>