数字农科院2.0

Effects Of Rnai-Based Silencing Of C.hitin Synthase Gene On M oulting And Fecundity In Pea Aphids (Acyrthosiphon Pisum)

文献类型： 外文期刊

作者： Niu, Jinzhi;Jiang, Yi-Di;Jiang, Yi-Di;Shang, Feng;An, Xin;An, Xin;Niu, Jinzhi;Wang, Jin-Jun;Wang, Jin-Jun;Shang, Feng;Ye, Chao;Ye, Chao;Yang, Li;Yang, Li

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期刊名称： SCIENTIFIC REPORTS

ISSN： 2045-2322

年卷期： 2019 年 9 卷

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摘要： The pea aphid, Acyrthosiphon pisum, is an important agricultural pest and an ideal model organism for various studies. Chitin synthase (CHS) catalyses chitin synthesis, a critical structural component of insect exoskeletons. Here, we identified a CHS gene from A. pisum, ApisCHS. The ApisCHS expression profiles showed that ApisCHS was expressed in various developmental stages and in all tested tissues of A. pisum, including the epidermis, embryo, gut and haemolymph. Notably, ApisCHS exhibited peak expression in the middle of each nymphal period and was extremely highly expressed in the epidermis and embryo. RNA interference (RNAi) showed that similar to 600 ng of dsRNA is an effective dose for gene silencing by injection for dsRNA delivery; moreover, 1200 ng.mu L-1 dsRNA induced CHS gene silencing by a plant-mediated feeding approach. A 44.7% mortality rate and a 51.3% moulting rate were observed 72 h after injection of dsApisCHS into fourth-instar nymphs, compared with the levels in the control (injected with dsGFP). Moreover, a longer period was required for nymph development and a 44.2% deformity rate among newborn nymphs was obtained upon ingestion of dsApisCHS. These results suggest that ApisCHS plays a critical role in nymphal growth and embryonic development in pea aphids, and is a potential target for RNAi-based aphid pest control.

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