数字农科院2.0

Proteomic Analysis Of The Resistance M.echanisms In Sugarcane During S porisorium Scitamineum Infection

文献类型： 外文期刊

作者： Yang, Li-Tao;Verma, Krishan Kumar;Song, Qi-Qi;Solanki, Manoj Kumar;Singh, Rajesh Kumar;Singh, Rajesh Kumar;Singh, Pratiksha;Malviya, Mukesh Kumar;Malviya, Mukesh Kumar;Li, Yang-Rui;Li, Yang-Rui;Li, Yang-Rui;Li, Hai-Bi;Verma, Krishan Kumar

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关键词： sugarcane; Sporisorium scitamineum; smut; proteomics; RT-qPCR; ISR

期刊名称： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

ISSN： 1422-0067

年卷期： 2019 年 20 卷 3 期

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摘要： Smut disease is caused by Sporisorium scitamineum, an important sugarcane fungal pathogen causing an extensive loss in yield and sugar quality. The available literature suggests that there are two types of smut resistance mechanisms: external resistance by physical or chemical barriers and intrinsic internal resistance mechanisms operating at host-pathogen interaction at cellular and molecular levels. The nature of smut resistance mechanisms, however, remains largely unknown. The present study investigated the changes in proteome occurring in two sugarcane varieties with contrasting susceptibility to smutF134 and NCo310at whip development stage after S. scitamineum infection. Total proteins from pathogen inoculated and uninoculated (control) leaves were separated by two-dimensional gel electrophoresis (2D-PAGE). Protein identification was performed using BLASTp and tBLASTn against NCBI nonredundant protein databases and EST databases, respectively. A total of thirty proteins spots representing differentially expressed proteins (DEPs), 16 from F134 and 14 from NCo310, were identified and analyzed by MALDI-TOF/TOF MS. In F134, 4 DEPs were upregulated and nine were downregulated, while, nine were upregulated and three were downregulated in NCo310. The DEPs were associated with DNA binding, metabolic processes, defense, stress response, photorespiration, protein refolding, chloroplast, nucleus and plasma membrane. Finally, the expression of CAT, SOD, and PAL with recognized roles in S. scitamineum infection in both sugarcane verities were analyzed by real-time quantitative PCR (RT-qPCR) technique. Identification of genes critical for smut resistance in sugarcane will increase our knowledge of S. scitamineum-sugarcane interaction and help to develop molecular and conventional breeding strategies for variety improvement.

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