数字农科院2.0

The Establishment Of Clonally Derived C.hicken Embryonic Fibroblast Cell L ine (Csc) With High Transfection Efficiency And Ability As A Feeder Cell

文献类型： 外文期刊

作者： Li, Bichun;Zuo, Qisheng;Ahmed, Mahmoud F.;Chen, Guohong;Li, Bichun;Zuo, Qisheng;Zhang, Yani;Zhao, Zhenhua;Zhang, Yani;Zuo, Qisheng;Zhao, Ruifeng;Wang, Man;Sun, Xinyu;Jin, Kai;Chen, Guohong;Li, Bichun;Zhang, Yani;Chen, Guohong;Jin, Jing

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关键词： chicken embryonic fibroblast; feed layer cells; mouth pipette; single clone; transfection

期刊名称： JOURNAL OF CELLULAR BIOCHEMISTRY

ISSN： 0730-2312

年卷期： 2018 年 119 卷 11 期

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摘要： This study established a single cloned chicken embryonic fibroblast (CEF) cell line. It solves the main problem of the instability of a cultured primary cell and its impact on the experiment. In this study, CEF pass through this crisis and formed a continuous cell line after subculture. We isolated single postcrisis CEF by a mouth pipette under a convert microscope then established a single cloned cell line named CSC-1-5 which passaged continuously from 96-well plates to 60mm culture plates. CSC has a normal chicken diploid karyotype, no tumorigenicity, and a high G2/M phase cell ratio. We found that Fugene could mediate the transfection of CSCs efficiently; it was significantly improved compared with the primary cells. It could also promote the proliferation of chicken embryonic stem cell as a feeder layer.

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