数字农科院2.0

Rig-I Is Responsible For Activation O.f Type I Interferon P athway In Seneca Valley Virus-Infected Porcine Cells To Suppress Viral Replication

文献类型： 外文期刊

作者： Li, PF; Zhang, XL; Cao, WJ; Yang, F; Du, XL; Shi, ZW; Zhang, MT; Liu, XT; Zhu, ZX; Zheng, HX

作者机构：

关键词： RIG-I; SVV; Interferon; Immune response; Viral replication

期刊名称： VIROLOGY JOURNAL

ISSN： 1743-422X

年卷期： 2018 年 15 卷

页码：

收录情况： JCR（2021版）

摘要： BackgroundRetinoic acid-inducible gene I (RIG-I) is a key cytosolic receptor of the innate immune system. Seneca valley virus (SVV) is a newly emerging RNA virus that infects pigs causing significant economic losses in pig industry. RIG-I plays different roles during different viruses infections. The role of RIG-I in SVV-infected cells remains unknown. Understanding of the role of RIG-I during SVV infection will help to clarify the infection process of SVV in the infected cells.MethodsIn this study, we generated a RIG-I knockout (KO) porcine kidney PK-15 cell line using the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated protein-9 nuclease (Cas9) genome editing tool. The RIG-I gene sequence of RIG-I KO cells were determined by Sanger sequencing method, and the expression of RIG-I protein in the RIG-I KO cells were detected by Western bloting. The activation status of type I interferon pathway in Sendai virus (SeV)- or SVV-infected RIG-I KO cells was investigated by measuring the mRNA expression levels of interferon (IFN)- and IFN-stimulated genes (ISGs). The replicative state of SVV in the RIG-I KO cells was evaluated by qPCR, Western bloting, TCID50 assay and indirect immunofluorescence assay.ResultsGene editing of RIG-I in PK-15 cells successfully resulted in the destruction of RIG-I expression. RIG-I KO PK-15 cells had a lower expression of IFN- and ISGs compared with wildtype (WT) PK-15 cells when stimulated by the model RNA virus SeV. The amounts of viral RNA and viral protein as well as viral yields in SVV-infected RIG-I WT and KO cells were determined and compared, which showed that knockout of RIG-I significantly increased SVV replication and propagation. Meanwhile, the expression of IFN- and ISGs were considerably decreased in RIG-I KO cells compared with that in RIG-I WT cells during SVV infection.ConclusionAltogether, this study indicated that RIG-I showed an antiviral role against SVV and was essential for activation of type I IFN signaling during SVV infection. In addition, this study suggested that the CRISPR/Cas9 system can be used as an effective tool to modify cell lines to increase viral yields during SVV vaccine development.

分类号：

  • 相关文献

[1]PCV2-VLP和SVV全病毒灭活的组合物对小鼠的免疫原性研究. 段卫同,王海伟,孙明霞,王尚辉,王刚,于力,蔡雪辉,涂亚斌. 2021

[2]非RIG-I依赖的鸡STING介导干扰素产生的信号通路. 程玉强,孙英杰,王恒安,严亚贤,丁铲,孙建和. 2015

[3]猪RIG-I真核表达载体的构建及其抗口蹄疫病毒作用研究. 王国庆,朱紫祥,曹伟军,杨帆,毛箬青,李丹,刘磊,郑海学. 2015

[4]北京鸭RIG-I基因的克隆及其生物信息学分析. 王同淑,李传峰,陈宗艳,孟春春,吴润. 2015

[5]RIG-I遗传修饰鸡模型的抗病毒天然免疫机制研究. 孟庆文,周长良,王伟,张雅春,陈洪岩. 2015

[6]敲除RIG-I基因流感病毒高产MDCK细胞系的建立及功能研究. 曾为俊,许曼,王辉,鲁国涛,邵玉乐,陈洪岩,刘建华,孟庆文. 2019

[7]Muscovy duck retinoic acid-induced gene I (MdRIG-I) functions in innate immunity against H9N2 avian influenza viruses (AIV) infections. Cheng, Yuqiang,Huang, Qingqing,Ji, Wenhui,Du, Bin,Fu, Qiang,An, Huiting,Wang, Hengan,Yan, Yaxian,Ding, Chan,Sun, Jianhe,Ding, Chan,Li, Jing.

[8]Augmented atherogenesis in ApoE-null mice co-exposed to polychlorinated biphenyls and 2,3,7,8-tetrachlorodibenzo-p-dioxin. Shan, Qiuli,Wang, Jing,Huang, Fengchen,Du, Yuguo,Lv, Xiaowen,Ma, Min. 2014

[9]New role of gramicidin A in RIG-I-like receptors-mediated IFN signalling. Li, Xiao,Sun, Xinyuan,Yan, Zhongyi,Zhao, Zhenxiang,Pang, Zhaojun,Yang, Han,Ji, Xiaoxin,Lei, Yi,Zhu, Zixiang,Guo, Xiangqian,Mu, Xin. 2023

[10]Duck Rig-I Restricts Duck Enteritis V.irus Infection. Huo, H, Wang, Y, Wang, DF, Wang, YP, Chen, XH, Zhao, LL, Chen, HY. 2019

[11]Type I Interferon Induced And A.ntagonized By Foot-And-Mouth Disease V irus. Ma, XX,Ma, LN,Chang, QY,Ma, P,Li, LJ,Wang, YY,Ma, ZR,Cao, X. 2018

[12]African Swine Fever Virus Regulates Host Energy and Amino Acid Metabolism To Promote Viral Replication. Qiao Xue,Huisheng Liu,Zixiang Zhu,Fan Yang,Yingying Song,Zongqiang Li,Zhaoning Xue,Weijun Cao,Xiangtao Liu,Haixue Zheng. 2022

[13]The Variation of Duck RIG-I-Mediated Innate Immune Response Induced by Different Virulence Avian Influenza Viruses. Boyu Zhai,Lanlan Liu,Xiang Li,Xinru Lv,Jinyan Wu,Jing Li,Shengze Lin,Yuxiang Yin,Jiaqi Lan,Jianan Du,Chenwei Wu,Yi Wen,Yajun Wang,Yulong Wang,Zhijun Hou,Yanbing Li,Hongliang Chai,Xiangwei Zeng. 2022

[14]Transcriptome Analysis of Retinoic Acid-Inducible Gene I Overexpression Reveals the Potential Genes for Autophagy-Related Negative Regulation. Shaotang Ye,Chen Tan,Xiaoyun Yang,Ji Wang,Qi Li,Liang Xu,Zhen Wang,Jianwei Mao,Jingyu Wang,Kui Cheng,Aolei Chen,Pei Zhou,Shoujun Li. 2022

[15]Swine acute diarrhea syndrome coronavirus nucleocapsid protein antagonizes the IFN response through inhibiting TRIM25 oligomerization and functional activation of RIG-I/TRIM25. Zhang J.,Shi H.,Zhang L.,Feng T.,Chen J.,Zhang X.,Ji Z.,Jing Z.,Zhu X.,Liu D.,Yang X.,Zeng M.,Shi D.,Feng L.. 2024

[16]N6-methyladenosine within transmissible gastroenteritis virus genomic RNA inhibits its replication via efficient recognition by RNA sensor RIG-I. Chen, Jianing,Lin, Shengyu,Liu, Qianzi,Gao, Mengling,Wang, Zemei,Tang, Jiao,Cui, Yaru,Tan, Chen,Liu, Guangliang. 2025

[17]The Perk Arm Of The U.nfolded Protein Response Negatively R egulates Transmissible Gastroenteritis Virus Replication By Suppressing Protein Translation And Promoting Type I Interferon Production. Xue, M, Fu, F, Ma, YL, Zhang, X, Li, L, Feng, L, Liu, PH. 2018

[18]Molecular Cloning And Functional Characterization O.f Eleven Subtypes Of I nterferon-Alpha In Amur Tigers (Panthera Tigris Altaica). Zhao, HJ, Ma, J, Wang, Y, Liu, JJ, Shao, YZ, Li, JL, Jiang, GS, Xing, MW. 2017

[19]Basal Interferon Signaling And Therapeutic Use Of Interferons In Controlling Peste Des Petits Ruminants Virus Infection. Chang, QY, Guo, FC, Liu, JL, Zhang, DR, Feng, YP, Ma, XX, Shang, YJ. 2019

[20]Equine Myxovirus Resistance Protein 2 R.estricts Lentiviral Replication By B locking Nuclear Uptake Of Capsid Protein. Ji, S, Na, L, Ren, HL, Wang, YJ, Wang, XJ. 2018

作者其他论文 更多>>