文献类型: 外文期刊
作者: Zhang Li-juan;Lu Li-min;Cui Jin-jie;Zhu Xiang-zhen;Wang Chun-yi;Luo Jun-yu;Wang Li;Zhang Shuai
作者机构:
关键词: cotton-melon aphid; Cytb and 16S genes; haplotype; biotype; cotton field
期刊名称: JOURNAL OF INTEGRATIVE AGRICULTURE
ISSN: 2095-3119
年卷期: 2018 年 17 卷 9 期
页码:
摘要: Aphis gossypii is a globally distributed species and therefore has a highly variable life cycle. Populations of A. gossypii in northern China exhibit greater genotypic diversity and a broader host range, yet the details of life cycles of different biotypes is still unclear. In this study, the Cytb and 16S gene regions of A. gossypii collected from 5 common summer hosts and 4 primary hosts were analyzed. A total of 57 haplotypes were obtained from 1046 individual A. gossypii sequences. The sequence included 44 variable sites, 27 of which were parsimony informative sites and 17 of which were singleton variable sites. The most frequent 3 haplotypes were found in 896 individuals, representing a total of 85.7% of all individuals and 36 haplotypes were found in 1 individual. A neighbor-joining tree was constructed using 21 haplotypes that were found in more than 2 individuals. Considering the individual host plant, 5 biotypes were identified. Type 1 corresponded exactly to the cucurbit host-race and the other 4 biotypes were found as cotton host-races. Type 3 was the most abundant biotype in cotton fields in northern China.
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