数字农科院2.0

Optimization Of Agrobacterium Tumefaciens-Mediated Transformation S.ystems In Tea Plant ( Camellia Sinensis (L.) O. Kuntze)

文献类型： 外文期刊

作者： Chen, X.;Chen, C. S.;Li, X. H.;Lv, Q. R.;Wang, L.;Xu, Y. J.;Sun, K.;Hu, S. K.

作者机构：

关键词： Camellia sinensis; cotyledon callus; Agrobacterium tumefaciens; GUS transient expression

期刊名称： II ASIAN HORTICULTURAL CONGRESS

ISSN： 0567-7572

年卷期： 2018 年 1208 卷

页码：

摘要： The cotyledon callus of Camellia sinensis were used as the receptors for transformation by Agrobacterium tumefaciens EHA105 containing PS1aG-3. Some factors which affected the result of Agrobacterium-mediated transformation of C. sinensis were studied on the basis of GUS transient expression system. The optimum system of Agrobacterium-mediated transformation was that the cotyledon callus were pre-cultured for 3 d, and then infected for 15 min by EHA105 followed by 3 d co-culture in the dark on the YEB medium containing 150 mu mol L-1 AS. The transient expression rate of GUS gene was 62.6%. An appropriate time of delayed selective culture could improve the conversion rate of the tea callus. After delaying the selective culture for 3 d, infected callus were transferred into the differentiation medium and the root induction medium, both of which were supplemented with 100 mg L-1 Spectinomycin, in the end resistant seedlings of C. sinensis were obtained. The conversion rate was about 3.6%.

分类号：

  • 相关文献

[1]Effects Of The Wheat Udp-Glucosyltransferase G.ene Taugt-B2 On Agrobacterium-Mediated P lant Transformation. Zhou, XH, Wang, K, Du, LP, Liu, YW, Lin, ZS, Ye, XG. 2017

[2]Research Progress On Agrobacterium Tumefaciens-Based T.ransgenic Technology In Brassica R apa. Li, GL, Yue, LX, Li, F, Zhang, SF, Zhang, H, Qian, W, Fang, ZY, Wu, J, Wang, XW, Zhang, SJ, Sun, RF. 2018

[3]Terpenoid Esters Are The Major C.onstituents From Leaf Lipid D roplets Of Camellia Sinensis. Zhou, Xin,Chen, Xiaobing,Thelen, Jay J.,Zhou, Xin,Zhou, Xin,Zhou, Xin,Chen, Changsong,Zhang, Wenjing,Chen, Mingjie,Chen, Xiaobing,Chen, Xiaobing,Chen, Xiaobing,Kong, Xiangrui,Du, Zhenghua,Zhang, Yi. 2019

[4]Exogenous Melatonin Enhances Cold, Salt A.nd Drought Stress Tolerance B y Improving Antioxidant Defense In Tea Plant (Camellia Sinensis (L.) O. Kuntze). Li, Jiahao,Chen, Yi,Chen, Xuan,Sun, Kang,Li, Xinghui,Yang, Yiqing. 2019

[5]Bioavailability Of Tea Catechins And I.ts Improvement. Cai, Zhuo-Yu,Li, Xu-Min,Xiang, Li-Ping,Shi, Yun-Long,Liang, Jin-Pei,Shi, Meng,Ye, Jian-Hui,Zheng, Xin-Qiang,Yang, Rui,Wang, Kai-Rong,Liang, Yue-Rong,Lu, Jian-Liang. 2018

[6]De Novo Transcriptome Combined With S.pectrophotometry And Gas Chromatography-Mass S pectrometer (Gc-Ms) Reveals Differentially Expressed Genes During Accumulation Of Secondary Metabolites In Purple-Leaf Tea (Camellia Sinensis Cv Hongyafoshou). Guo, Yuqiong,Chang, Xiaojun,Chang, Xiaojun,Zhang, Shuting,Zhang, Shuting,Zhu, Chen,Lin, Yuling,Lai, Zhongxiong,Zhu, Chen,Li, Xiaozhen,Lin, Yuling,Fu, Haifeng,Chen, Changsong,Lai, Zhongxiong. 2019

[7]Integrated Transcriptomic And Phytochemical Analyses P.rovide Insights Into Characteristic M etabolites Variation In Leaves Of 1-Year-Old Grafted Tea (Camellia Sinensis). Wang, Rangjian,Wan, Xiaochun,Zhang, Zheng-Zhu,Zhu, Biying,Lu, Mengqian,Han, Jieyun,Deng, Wei-Wei. 2019

[8]Domestication Origin And Breeding History O.f The Tea Plant ( &Itcamellia&It &Itsinensis&It) In China And India Based On Nuclear Microsatellites And Cpdna Sequence Data. Liu, Jie,Wambulwa, Moses C.,Liu, Ben-Ying,Li, Miao-Miao,Li, De-Zhu,Li, De-Zhu,Gao, Lian-Ming,Meegahakumbura, Muditha K.,Li, De-Zhu,Li, Miao-Miao,Sun, Yong-Shuai,Wambulwa, Moses C.,Moller, Michael,Meegahakumbura, Muditha K.,Meegahakumbura, Muditha K.,Wambulwa, Moses C.,Xu, Jian-Chu,Yang, Jun-Bo,Wambulwa, Moses C.,Meegahakumbura, Muditha K.,Thapa, Kishore K.. 2018

[9]Light-Sensitive Albino Tea Plants And T.heir Characterization. Shi, Yun-Long,Liang, Yue-Rong,Ma, Shi-Cheng,Zheng, Xin-Qiang,Lu, Jian-Liang,Fu, Qiu-Yue,Shin, Young-Hwan,Wang, Kai-Rong,Ye, Jian-Hui,Li, Xu-Min,Yang, Rui. 2018

[10]Uncovering Tea-Specific Secondary Metabolism Using T.ranscriptomic And Metabolomic Analyses I n Grafts Of Camellia Sinensis And C. Oleifera. Deng, Wei-Wei,Wan, Xiaochun,Zhang, Zheng-Zhu,Zhu, Biying,Tai, Yuling,Lu, Mengqian,Han, Jieyun,Wang, Rangjian,Fan, Yanbing. 2018

[11]Overexpression Of A Camellia Sinensis D.reb Transcription Factor Gene ( Csdreb) Increases Salt And Drought Tolerance In Transgenic Arabidopsis Thaliana. Wang, Mingle,Xin, Huahong,Li, Xinghui,Zou, Zhongwei,Li, Qinghui,Zhuang, Jing. 2017

[12]Heterologous Expression Of Three Camellia S.inensis Small Heat Shock P rotein Genes Confers Temperature Stress Tolerance In Yeast And Arabidopsis Thaliana. Zhu, Xujun,Xin, Huahong,Li, Xinghui,Li, Qinghui,Chen, Xuan,Zou, Zhongwei,Wang, Mingle. 2017

[13]Involvement Of Cscdpk20 And Cscdpk26 I.n Regulation Of Thermotolerance I n Tea Plant (Camellia Sinensis). Wang, Mingle,Li, Qinghui,Sun, Kang,Chen, Xuan,Zhou, Qiongqiong,Li, Huan,Li, Xinghui,Wang, Mingle,Zhang, Xuyang,Li, Qinghui. 2018

[14]Developmental changes in carbon and nitrogen metabolism affect tea quality in different leaf position. Li, Zhi-Xin,Ahammed, Golam Jalal,Shen, Chen,Yan, Peng,Li, Xin,Han, Wen-Yan,Li, Zhi-Xin,Shen, Chen,Yang, Wei-Jun,Ahammed, Golam Jalal.

[15]Altitudinal effects on the quality of green tea in east China: a climate change perspective. Han, Wen-Yan,Li, Xin,Li, Zhi-Xin,Ahammed, Golam Jalal,Yan, Peng,Huang, Ji-Gang,Stepp, John Richard.

[16]Variability and stability of tea weevil-induced volatile emissions from tea plants with different weevil densities, photoperiod and infestation duration. Cai, Xiao-Ming,Sun, Xiao-Ling,Dong, Wen-Xia,Wang, Guo-Chang,Chen, Zong-Mao,Cai, Xiao-Ming,Wang, Guo-Chang. 2012

[17]Development and characterization of single nucleotide polymorphism markers in Camellia sinensis (Theaceae). Zhang, C. C.,Wang, L. Y.,Wei, K.,Cheng, H.. 2014

[18]Seasonal climate effects on flavanols and purine alkaloids of tea (Camellia sinensis L.). Wang, L. Y.,Wei, K.,Jiang, Y. W.,Cheng, H.,Zhou, J.,He, W.,Zhang, C. C.. 2011

[19]Simultaneous HPLC Determination of Amino Acids in Tea Infusion Coupled to Pre-column Derivatization with 2,4-Dinitrofluorobenzene. Li, Nana,Liu, Yang,Zhao, Yue,Zheng, Xinqiang,Lu, Jianliang,Liang, Yuerong,Li, Nana.

[20]Cloning and Characterization of a Flavonoid 3'-Hydroxylase Gene from Tea Plant (Camellia sinensis). Zhou, Tian-Shan,Yu, You-Ben,Xiao, Yao,Li, Dong-Hua,Xiao, Bin,Zhou, Rui,Yu, Oliver,Yang, Ya-Jun. 2016

作者其他论文 更多>>