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Biological Characteristics Of A New A.ntibacterial Peptide And Its A ntibacterial Mechanisms Against Gram-Negative Bacteria

文献类型： 外文期刊

作者： Pei, Z.;Ying, X.;Tang, Y.;Kong, L.;Ma, H.;Liu, S.;Zhang, H.;Liu, L.;Wang, K.;Zhang, D.;Gao, Y.

作者机构：

关键词： antimicrobial peptide; biological characteristics; antibacterial mechanism; Gram-negative bacteria

期刊名称： POLISH JOURNAL OF VETERINARY SCIENCES

ISSN： 1505-1773

年卷期： 2018 年 21 卷 3 期

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摘要： MDAP-2 is a new antibacterial peptide with a unique structure that was isolated from houseflies. However, its biological characteristics and antibacterial mechanisms against bacteria are still poorly understood. To study the biological characteristics, antibacterial activity, hemolytic activity, cytotoxicity to mammalian cells, and the secondary structure of MDAP-2 were detected; the results showed that MDAP-2 displayed high antibacterial activity against all of the tested Gram-negative bacteria. MDAP-2 had lower hemolytic activity to rabbit red blood cells; only 3.4% hemolytic activity was observed at a concentration of 800 mu g/ml. MDAP-2 also had lower cytotoxicity to mammalian cells; IC50 values for HEK-293 cells, VERO cells, and IPEC-J2 cells were greater than 1000 mu g/ml. The circular dichroism (CD) spectra showed that the peptide mostly has alpha-helical properties and some beta-fold structure in water and in membrane-like conditions. MDAP-2 is therefore a promising antibacterial agent against Gram-negative bacteria. To determine the antibacterial mechanism(s) of action, fluorescent probes, flow cytometry, and transmission electron microscopy (TEM) were used to study the effects of MDAP-2 on membrane permeability, polarization ability, and integrity of Gram-negative bacteria. The results indicated that the peptide caused membrane depolarization, increased membrane permeability, and destroyed membrane integrity. In conclusion, MDAP-2 is a broad-spectrum, lower hemolytic activity, and lower cytotoxicity antibacterial peptide, which is mainly effective on Gram-negative bacteria. It exerts its antimicrobial effects by causing bacterial cytoplasm membrane depolarization, increasing cell membrane permeability and disturbing the membrane integrity of Gram-negative bacteria. MDAP-2 may offer a new strategy to for defense against Gram-negative bacteria.

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