数字农科院2.0

Topical Dsrna Delivery Induces Gene S.ilencing And Mortality In T he Pea Aphid

文献类型： 外文期刊

作者： Tian, Yuan;Fan, Jia:Yao;Wang, Jin:Jun;Niu, Jinzhi;Yang, Wan:Jun;Tian, Yuan;Ding, Bi:Yue;Yang, Wan:Jun;Fan, Jia:Yao;Ye, Chao;Zhang, Jun;Ding, Bi:Yue;Shang, Feng;An, Xin;Yang, Li;Chang, Teng:Yu;Ye, Chao;Smagghe, Guy;Christiaens, Olivier;Zhang, Jun;Yang, Li;Niu, Jinzhi;An, Xin;Wang, Jin:Jun;Smagghe, Guy;Shang, Feng;Smagghe, Guy;Chang, Teng:Yu

作者机构：

关键词： Acyrthosiphon pisum; Aphis citricidus; Myzus persicae; topical RNAi; RNAi efficiency

期刊名称： PEST MANAGEMENT SCIENCE

ISSN： 1526-498X

年卷期： 2019 年 75 卷 11 期

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摘要： BACKGROUND With the growing number of available aphid genomes and transcriptomes, an efficient and easy-to-adapt tool for gene function study is urgently required. RNA interference (RNAi), as a post-transcriptional gene silencing mechanism, is important as a research tool for determining gene functions and has potential as a novel insect control strategy. However, these applications have been hampered by the lack of effective dsRNA delivery approaches in aphids. RESULTS Here, we developed a convenient and efficient dsRNA delivery method, topical RNAi, in aphids. An investigation of its dose and time-dependent RNAi efficiencies revealed that with as little as 60 ng dsRNA per adult pea aphid (Acyrthosiphon pisum), the indicator gene, Aphunchback, could be significantly silenced within 2 h of exposure. The method was further validated by successfully silencing other different genes, and it was also efficient toward two other aphid species, Aphis citricidus and Myzus persicae. Furthermore, a noticeable mortality was also observed in pea aphids using topical RNAi-mediated gene silencing, within 4 days post-dsRNA application for four out of seven tested genes. CONCLUSION Compared with the currently used dsRNA delivery methods in aphids, microinjection and ingestion, topical RNAi is time- and cost-effective, which could greatly influence RNAi-based gene functional studies and potential candidate gene selection for developing RNAi-based aphid control strategies in the future. (c) 2019 Society of Chemical Industry

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