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Complete Nucleotide Sequence Of Loquat V.irus A, A Member O f The Family Betaflexiviridae With A Novel Genome Organization

文献类型: 外文期刊

作者: Liu, Qiyan;Qiu, Yuanjian;Zhang, Song;Zhou, Changyong;Xuan, Zhiyou;Yang, Liu;Xuan, Zhiyou;Cao, Mengji;Wu, Jiaxing;Wu, Jiaxing;Liu, Qiyan;Yang, Liu;Cao, Mengji;Wu, Di;Zhou, Changyong;Qiu, Yuanjian;Zhang, Song

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期刊名称: ARCHIVES OF VIROLOGY

ISSN: 0304-8608

年卷期: 2019 年

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摘要: Analysis of a loquat tree with leaf curl symptoms by deep sequencing revealed a novel virus with a single-stranded RNA genome, for which the name "loquat virus A" (LoVA) was proposed. The complete genome sequence comprised 7553 nucleotides (nt) and an additional poly(A) tail at the 3 ' terminus. Sequence comparisons of LoVA showed moderate similarity to cherry virus A (CVA), currant virus A (CuVA), and mume virus A (MuVA), which are members of the genus Capillovirus in the family Betaflexiviridae. Phylogenetic analysis of the full-genome nt sequence and replicase-like protein supported the placement of LoVA within the genus Capillovirus. However, it has a distinct genome organization, differing from recognized capilloviruses, as it contains three open reading frames (ORFs), with the coat protein (CP) expressed separately from the replication-associated protein (RP) rather than being encoded in the same ORF. This indicates that LoVA is a novel member of the genus Capillovirus in the family Betaflexiviridae with a distinct genomic organization.

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