数字农科院2.0

Identification Of Chicken Meat Quality V.ia Rapid Array Isoelectric F ocusing With Extraction Of Hemoglobin And Myoglobin In Meat Sample

文献类型： 外文期刊

作者： Saud, S; Li, GQ; Kong, H; Khan, MI; Qiang, Z; Sun, YJ; Liu, WW; Ding, C; Xiao, H; Wang, YX; Li, HG; Cao, CX

作者机构：

关键词： Array isoelectric focusing; Food safety; Hemoglobin; Meat quality; Myoglobin

期刊名称： JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES

ISSN： 1570-0232

年卷期： 2019 年 1128 卷

页码：

收录情况： JCR（2021版） ; EI（2021版）

摘要： Isoelectric focusing (IEF) has been used for determination of meat quality with high stability analysis. However, it still suffered from time-consuming, laborious and cost-effective performances, e.g., 3 h protein extraction, more than 10 h rehydration time, 5-12 h focusing time, and imaging of protein band. To overcome these issues, a speedy extraction of colorful proteins was developed by controlling extraction and centrifugation of 0.2g sample within 10 min and 15 min respectively; a rapid analytical method was designed by using a quick array IEF with 25 min rehydration, 7 min focusing, 2 min online scanning and imaging of focused proteins. The total analytical time was well controlled within 1 h, significantly less than the traditional IEF time of 24 h. To demonstrate the proposed method, 18 chickens were classified into three groups, e.g., the normal slaughtering, death treatment underwater, and death with infection via the New castle disease (NDV) virus. The experiments demonstrated that two Mb bands with pI 6.8 and 7.4 were present in slaughtered chickens, while four other bands with pI 6.83, 6.95, 7.09, and 7.13 were observed inl abnormal chicken. The additional four proteins bands were identified by western blot (WB) as hemoglobin proteins. Furthermore, array Immobilized pH Gradient (IPG) has high sensitivity (absolute LOD of Mb and Hb were 1.3 ng and 5.5 ng), fair stability (RSD values of 2.32%, 2.27%, and 1.69%) for slaughtered, drowned, NDV-infected chickens for infra-day and (2.94%, 1.66%, and 1.07%) for interdays, and good recovery (100%, 98.25% and 99.75%). Finally, the developed method could be used for the identification of chicken meat quality with less time and small volume reagents consuming.

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