数字农科院2.0

Isoelectric Focusing On Microfluidic Paper-Based C.hips

文献类型: 外文期刊

作者: Yu, SY; Yan, CF; Hu, XQ; He, BQ; Jiang, Y; He, QH

作者机构:

关键词: Microfluidic paper-based chips; Isoelectric focusing; Protein; Separation

期刊名称: ANALYTICAL AND BIOANALYTICAL CHEMISTRY

ISSN: 1618-2642

年卷期: 2019 年 411 卷 21 期

页码:

收录情况: JCR(2021版) ; EI(2021版)

摘要: Isoelectric focusing (IEF), a powerful technique for protein separation and enrichment, was successfully integrated into microfluidic paper-based analytical devices (mu PADs) in this work. The mu PADs for isoelectric focusing were fabricated by octadecyltrichlorosilane (OTS) silanization and subsequent region-selective plasma treatment. The system of IEF on mu PADs could be easily assembled. And a series of conditions of the system were investigated, including the suitable concentration of ampholyte to create good pH gradient, the effect of polyvinylpyrrolidone (PVP) on electroosmotic flow (EOF) suppression, and focusing voltage applied on the paper channel. After optimization, simultaneous separation and enrichment of protein sample containing myoglobin and cytochrome C was successfully demonstrated. Besides, parallel IEF on multichannels were also achieved for the separation of multiple protein samples on one single chip, and their performance was compared with that of the conventional gel-IEF system. The developed IEF on mu PADs exhibits appealing features such as low cost, simplicity, and disposability and are believed to have great application potentials.

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