数字农科院2.0

Co-Immunization With Two Recombinant Eimeria T.enella Lines Expressing Immunoprotective A ntigens Of E. Maxima Elicits Enhanced Protection Against E. Maxima Infection

文献类型： 外文期刊

作者： Tang, XM; Wang, CY; Liang, L; Hu, DD; Zhang, SX; Duan, CH; Suo, JX; Liu, XY; Suo, X; Cui, SJ

作者机构：

关键词： Recombinant Eimeria; Vaccine vector; Apical membrane antigen 1; Immune mapped protein 1; Immune responses

期刊名称： PARASITES & VECTORS

ISSN： 1756-3305

年卷期： 2019 年 12 卷

页码：

收录情况： JCR（2021版）

摘要： BackgroundLive anticoccidial vaccines have been a tremendous success for disease prevention. The establishment of the reverse genetic manipulation platform has enabled the development of Eimeria parasites, the live anticoccidial vaccine strains, as vaccine vectors. In our previous study, recombinant E. tenella expressing a single immunodominant antigen of E. maxima (Et-EmIMP1) was able to protect chickens against challenge infection with E. maxima. This promising result encouraged us to further explore strategies to improve the protection efficacy of recombinant Eimeria and develop it as a vaccine vector.ResultsWe constructed a novel recombinant Eimeria line expressing apical membrane antigen 1 of E. maxima (Et-EmAMA1) and then immunized chickens with Et-EmAMA1 and/or Et-EmIMP1. We found that the E. maxima soluble antigen-specific cell-mediated immunity was much stronger in the birds that were co-immunized with Et-EmAMA1 and Et-EmIMP1 than in those that were immunized with Et-EmAMA1 or Et-EmIMP1 alone. The oocyst production after E. maxima infection was significantly reduced in the recombinant Eimeria-immunized birds compared with the wild-type-immunized and naive birds. The oocyst production in the birds co-immunized with Et-EmAMA1 and Et-EmIMP1 was consistently the lowest among the treatment groups after E. maxima infection.ConclusionsThese results demonstrated that Eimeria is an effective vaccine vector that can carry and deliver heterologous Eimeria antigens to the host immune system and trigger specific immune responses. Our results also suggested that increasing the number of recombinant Eimeria lines is an effective approach to enhance protective immunity against infections with heterologous pathogens.

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