数字农科院2.0

Dissecting A Heterotic Gene Through G.radedpool-Seq Mapping Informs A R ice-Improvement Strategy

文献类型： 外文期刊

作者： Xie, Huaan;Yang, Shihua;Zhan, Qilin;Wang, Ziqun;Wang, Changsheng;Wang, Wuqi;Wang, Yongchun;Zhao, Yan;Cui, Lingling;Zhao, Qiang;Zhou, Congcong;Zhang, Jianfu;Zhu, Jingjie;Zhu, Chuanrang;Wang, Changsheng;Hou, Qingqing;Han, Bin;Gong, Junyi;Hou, Qingqing;Lu, Yiqi;Li, Yan;Huang, Xuehui;Shangguan, Yingying;Tang, Shican;Tang, Shican;Miao, Jiashun;Feng, Qi;Lyu, Danfeng;Miao, Jiashun

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期刊名称： NATURE COMMUNICATIONS

ISSN： 2041-1723

年卷期： 2019 年 10 卷

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摘要： Hybrid rice breeding for exploiting hybrid vigor, heterosis, has greatly increased grain yield. However, the heterosis-related genes associated with rice grain production remain largely unknown, partly because comprehensive mapping of heterosis-related traits is still labor-intensive and time-consuming. Here, we present a quantitative trait locus (QTL) mapping method, GradedPool-Seq, for rapidly mapping QTLs by whole-genome sequencing of gradedpool samples from F-2 progeny via bulked-segregant analysis. We implement this method and map-based cloning to dissect the heterotic QTL GW3p6 from the female line. We then generate the near isogenic line NIL-FH676::GW3p6 by introgressing the GW3p6 allele from the female line Guangzhan63-4S into the male inbred line Fuhui676. The NIL-FH676::GW3p6 exhibits grain yield highly increased compared to Fuhui676. This study demonstrates that it may be possible to achieve a high level of grain production in inbred rice lines without the need to construct hybrids.

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