数字农科院2.0

Comprehensive Proteomic And Metabolomic Profiling O.f Mcr-1-Mediated Colistin Resistance I n Escherichia Coli

文献类型： 外文期刊

作者： Li, H; Wang, YY; Meng, QS; Wang, Y; Xia, GL; Xia, X; Shen, JZ

作者机构：

关键词： mcr-1; Colistin; Proteomic; Metabolomic; Antimicrobial resistance

期刊名称： INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS

ISSN： 0924-8579

年卷期： 2019 年 53 卷 6 期

页码：

收录情况： JCR（2021版）

摘要： Spread of the mcr-1 gene in human and veterinary medicine has jeopardised the use of polymyxins, last-resort antibiotics against life-threatening multidrug-resistant Gram-negative bacteria. As a lipid-modifying gene, whether mcr-1 causes proteomic and metabolomic changes in bacteria and affects the corresponding metabolic pathway is largely unknown. In this study, label-free quantitative proteomics and untargeted metabolomics were used to profile comprehensive proteome and metabolome characteristics of mcr-1-mediated colistin-resistant and-susceptible Escherichia coli in order to gain further insight into the colistin resistance mechanism. Large sets of differentially expressed proteins (DEPs) and metabolites were identified that contributed to mcr-1-mediated antimicrobial resistance, predominantly in different growth conditions with and without colistin. mcr-1 caused downregulated expression of most proteins in order to adapt to drug pressure. Pathway analysis showed that metabolic processes were significantly affected, mainly related to glycerophospholipid metabolism, thiamine metabolism and lipopolysaccharide (LPS) biosynthesis. The substrate phosphoethanolamine (PEA) for mcr-1 to mediate colistin resistance was accumulated in colistin-resistant E. coli. Notably, mcr-1 not only caused PEA modification of the bacterial cell membrane lipid A but also affected the biosynthesis and transport of lipoprotein in colistin resistance by disturbing the expression of efflux pump proteins involved in the cationic antimicrobial peptide (CAMP) resistance pathway. Overall, disturbed glycerophospholipid metabolism and LPS biosynthesis as well as accumulation of the substrate PEA was closely related with mcr-1-mediated colistin resistance. These findings could provide further valuable information to inhibit colistin resistance by blocking this metabolic process. (C) 2019 Elsevier B.V. and International Society of Chemotherapy. All rights reserved.

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