Optimization Of Ultrasonic-Microwave Synergistic Extraction O.f Flavonoids From Sweet P otato Leaves By Response Surface Methodology
文献类型: 外文期刊
作者: Liu, J; Mu, TH; Sun, HN; Fauconnier, ML
作者机构:
期刊名称: JOURNAL OF FOOD PROCESSING AND PRESERVATION
ISSN: 0145-8892
年卷期: 2019 年 43 卷 5 期
页码:
收录情况: JCR(2021版) ; EI(2021版)
摘要: Ultrasonic-microwave synergistic extraction was used to extract flavonoids from sweet potato leaves (SPL) by response surface methodology. The optimal conditions for flavonoids extraction were 1:40 (g/ml) of solid-liquid ratio, 57 degrees C of extraction temperature, 76s of extraction time, and 72% (v/v) ethanol for 2 times, the highest extraction efficiency was 91.653.37%. After purification, the flavonoids purity reached to 76.10 +/- 3.11 (%, DW). The result of high-performance liquid chromatography revealed 11 compounds including astragalin (473.8 +/- 7.3mg/g, DW), quercetrin (86.5 +/- 0.7mg/g, DW), 4,5-chlorogenic acid (76.4 +/- 0.5mg/g, DW), isoquercitrin (62.4 +/- 0.4mg/g, DW), tiliroside (18.8 +/- 0.3mg/g, DW), quercetin (12.5 +/- 0.2mg/g, DW), 3,4,5-chlorogenic acid (6.5 +/- 0.2mg/g, DW), caffeic acid (6.1 +/- 0.2mg/g, DW), kaempferol (6.0 +/- 0.2mg/g, DW), myricetrin (5.9 +/- 0.1mg/g, DW), and rhamnetin (4.3 +/- 0.1mg/g, DW) in sweet potato leaf flavonoids, which possessed good antioxidant activity compared to soy isoflavones, ginkgo biloba extract, and propolis flavone. The IC50 value of sweet potato leaf flavonoids was 13.26 +/- 0.09g/ml in ferric reducing antioxidant power and 5.41 +/- 0.21 in 2, 2-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) scavenging capacity, respectively. Practical applicationsChina is the leading country of sweet potato production in the world, but sweet potato leaves (SPL) have been neglected except for a partial use as livestock feed in most parts of China, and detailed reports on the effective components in the leaves are scarce. Ultrasonic-microwave synergistic extraction is an efficient way to select flavonoids from SPL which has a potential to be extended in natural flavonoids industry.
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