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Alanine-Substituted Mutant On Gly(373) And A.sn(375) Of Cry1Ai-H-Loop 2 C auses Reduction In Both Toxicity And Binding Against Helicoverpa Armigera

文献类型： 外文期刊

作者： Zhou Zi-shan;Song Fu-ping;Zhang Jie;Liang Ge-mei;Liu Yu-xiao

作者机构：

关键词： Bacillus thuringiensis; Cry1Ai; Domain II-loop2; Helicoverpa armigera; binding affinity

期刊名称： JOURNAL OF INTEGRATIVE AGRICULTURE

ISSN： 2095-3119

年卷期： 2019 年 18 卷 5.0 期

页码：

收录情况： JCR（2021版） ; CSCD（2020-2021年度） ; 农林核心（2020版） ; 科技核心（2020版）

摘要： Cry1Ai-h-loop 2 is a mutant of Cry1Ai constructed by exchanging loop 2 from Cry1Ah protein and shows insecticidal activity against Helicoverpa armigera. The toxicity of Cry1Ai-h-loop 2, in contrast to the very low toxicity of Cry1Ai, is closely associated with the eleven residues in the loop 2 region. To characterize the key sites of loop 2 in Cry1Ai-h-loop 2, alanine-substituted mutants were generated. The toxicity of these mutants against H. armigera indicated that dual-mutant on Gly(373) and Asn(375) caused a significant decrease in toxic activity. ELISA binding and competition binding assays demonstrated that the reduction of toxicity in the mutant of interest was correlated with decreased binding affinity.

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