数字农科院2.0

Efficient Characterization Of Tetraploid Watermelon

文献类型： 外文期刊

作者： Chen, Wei;Tang, Mi;Feng, Gang;Bao, Yaning;Yan, Chao;Sun, Yuhong;Huang, Xing;Xie, Zhouli;Xiong, Jianshun;Zeng, Hongxia;Zhang, Na;Li, Yuhua;Ren, Jian

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关键词： Watermelon; Diploid; Tetraploid; Flow Cytometry; Qpcr

期刊名称： PLANTS-BASEL

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年卷期： 2019 年 8 卷 10 期

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摘要： Watermelon (Citrullus lanatus (Thunb.) Matsum. &Nakai) is an economic crop, which is widely cultivated around the world. The ploidy study of watermelon has an important role in field breeding and production, therefore, timely and convenient ploidy detection is necessary to accelerate its application. Traditionally, the ploidy of watermelon was determined by a series of time-consuming, expensive, and less efficient methods. In this study, we developed a more efficient method to simplify and accelerate the polyploidy identification in watermelons. We first confirmed the ploidy of watermelon by traditional tetraploid morphological features and well-established flow cytometry (FCM). Then we developed a reliable real-time quantitative PCR (qPCR) technique by quantifying the highly conserved 5S rDNA sequence and its copy numbers. This technique requires less sample collection and has comparable accuracy to FCM, it accelerates the analysis process and provides a new method for the identification of polyploidy of watermelon.

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