数字农科院2.0

Characterization Of A Novel Mycobacterium Tuberculosis Serine Protease (Rv3194C) Activity And Pathogenicity

文献类型： 外文期刊

作者： Li, H; Dang, GH; Liu, HX; Wang, ZX; Cui, ZY; Song, NN; Chen, LP; Liu, SG

作者机构：

关键词： Mycobacterium Tuberculosis; Serine Protease; Rv3194C; Activity And Pathogenicity; Mycobacterium Smegmatis

期刊名称： TUBERCULOSIS

ISSN： 1472-9792

年卷期： 2019 年 119 卷

页码：

收录情况： JCR（2021版）

摘要： Mycobacterium tuberculosis (MTB) serine proteases are important pathogen-associated virulence factors that are involved in the invasion, bacterial persistence, and degradation of host defense factors. The current study identified and characterized a novel serine protease, Rv3194c, of MTB. A hetemlogous Rv3194c protein, purified from Escherichia coli, possessed proteolytic activity that could hydrolyze bovine serum albumin (BSA), milk, casein, and gelatin at an optimal temperature of 40 degrees C and a pH of 8.0. Furthermore, the divalent metal ions Ca2+ and Mn2+ increased the activity of Rv3194c. Betulinic acid, a Traditional Chinese Medicine (TCM) monomer; PMSF, a chemical inhibitor; and the Roche inhibitor cocktail inhibited proteolytic activity. Site-directed mutagenesis demonstrated that D308 and particularly 5309 play a crucial role in the catalytic activity of Rv3194c protease. The cellular assays revealed that Rv3194c inhibits THP1-derived macrophage migration. Moreover, Rv3194c degraded the complement components, C3b and C5a, causing inhibition of phagocytosis and chemotaxis. In mice, Rv3194c enhanced the persistence of Mycobacterium smegmatis (Ms) in the lung, induced lung lesions, and promoted the release of inflammatory cytokines. The results of this study indicate that Rv3194c may play an important role in the pathogenicity of mycobacteria.

分类号：

  • 相关文献

[1]Characterization And Pathogenicity Of Extracellular Serine Protease Map3292C From Mycobacterium Avium Subsp. Paratuberculosis. Liu, HX, Dang, GH, Zang, XX, Cai, ZM, Cui, ZY, Song, NN, Liu, SG. 2020

[2]Pe17 Protein From Mycobacterium Tuberculosis E.nhances Mycobacterium Smegmatis Survival I n Macrophages And Pathogenicity In Mice. Li, Z, Liu, HX, Li, HF, Dang, GH, Cui, ZY, Song, NN, Wang, QK, Liu, SG, Chen, LP. 2019

[3]Characterization of Rv0394c gene encoding hyaluronidase and chondrosulfatase from Mycobacterium tuberculosis. Yuan, Xianglian,Chen, Liping,Cao, Jun,Yu, Shenye,Liu, Siguo,Yuan, Xianglian,Deng, Xiaoxia,Pang, Hai,Wang Quankai. 2013

[4]Development of one-tube multiplex polymerase chain reaction (PCR) for detecting Mycobacterium bovis. Quan, Zhang,Haiming, Tan,Xiaoya, Cai,Weifeng, Yuan,Hong, Jia,Hongfei, Zhu.

[5]Crystallization and preliminary X-ray analysis of a novel esterase Rv0045c from Mycobacterium tuberculosis. Xu, Lipeng,Liu, Siguo,Xu, Lipeng,Guo, Jiubiao,Zheng, Xiangdong,Pang, Hai,Wen, Tingyi,Sun, Fei. 2010

[6]Expression, Purification and Characterisation of Secreted Esterase Rv2525c from Mycobacterium tuberculosis. Dang, Guanghui,Chen, Liping,Li, Zhaoli,Cui, Yingying,Cao, Jun,Yu, Shenye,Liu, Siguo,Deng, Xiaoxia,Pang, Hai.

[7]C-terminal heat shock protein 70 of Mycobacterium tuberculosis as a molecular adjuvant for DNA vaccination with the porcine circovirus type 2 ORF2 (capsid) gene in mice. Fu, Fang,Tian, Huabin,Li, Xuesong,Lang, Yuekun,Liu, Siguo,Li, Haizhong,Wang, Wei,Li, Xi,Chen, Xin,Li, Xuesong,Tong, Guangzhi. 2013

[8]A novel B cell epitope in cold-shock DEAD-box protein A from Mycobacterium tuberculosis. Wang, Huanan,Zhu, Ting,Yu, Shenye,Liu, Huifang,Wang, Xiumei,Chen, Liping,Si, Wei,Liu, Siguo,Wang, Huanan,Pang, Hai.

[9]Characterization of a novel exported esterase Rv3036c from Mycobacterium tuberculosis. Chen, Liping,Dang, Guanghui,Cao, Jun,Yu, Shenye,Liu, Siguo,Dang, Guanghui,Deng, Xiaoxia,Pang, Hai,Dang, Guanghui,Wu, Defeng.

[10]A VLP-Based Vaccine Displaying HBHA and MTP Antigens of Mycobacterium tuberculosis Induces Protective Immune Responses in M. tuberculosis H37Ra Infected Mice. Juan Wang,Tao Xie,Inayat Ullah,Youjun Mi,Xiaoping Li,Yang Gong,Pu He,Yuqi Liu,Fei Li,Jixi Li,Zengjun Lu,Bingdong Zhu. 2023

[11]Extracellular Sphingomyelinase Rv0888 Of Mycobacterium T.uberculosis Contributes To Pathological L ung Injury Of Mycobacterium Smegmatis In Mice Via Inducing Formation Of Neutrophil Extracellular Traps. Dang, GH, Cui, YY, Wang, L, Li, TT, Cui, ZY, Song, NN, Chen, LP, Pang, H, Liu, SG. 2018

[12]PE12 interaction with TLR4 promotes intracellular survival of Mycobacterium tuberculosis by suppressing inflammatory response. Jiajun Zhang,Yingying Cui,Xinxin Zang,Tingting Feng,Fanruo Chen,Hui Wang,Guanghui Dang,Siguo Liu. 2023

[13]The combination of Mycobacterium tuberculosis fusion proteins LT33 and LT28 induced strong protective immunity in mice. Pu He,Juan Wang,Daquan Tan,Lina Hu,Yanlin Ma,Youjun Mi,Fei Li,Tingting Zhang,Yunjie Du,Wenhua Zhang,Jixi Li,Lei Jiao,Bingdong Zhu. 2024

[14]Plasma-based ultrasensitive detection of Mycobacterium tuberculosis ESAT6/CFP10 fusion antigen using a CRISPR-driven aptamer fluorescence testing (CRAFT). Siyuan Liu,Guohui Xiao,Peixuan Li,Yating Xu,Xinhao Fan,Lin Tian,Su Zhang,Guoliang Zhang. 2025

[15]Deep Amplicon Sequencing Reveals Culture-dependent Clonal Selection of Mycobacterium tuberculosis in Clinical Samples. Jiuxin Qu,Wanfei Liu,Shuyan Chen,Chi Wu,Wenjie Lai,Rui Qin,Feidi Ye,Yuanchun Li,Liang Fu,Guofang Deng,Lei Liu,Qiang Lin,Peng Cui. 2025

[16]Development and application of a curcumin-cinnamon essential oil nanoemulsion agent against mycobacteria. Lei, Zhihui,Ren, Yixuan,Wang, Jinyao,Shi, Haisu,Lin, Hu,Chen, Hong,Bi, Lijun,Wang, Yutang,Zhang, Hongtai. 2025

[17]Cross-sectional analysis of tuberculosis burden and risk factors in Swabi district, Khyber Pakhtunkhwa, Pakistan. Iqbal Ahmad,Madiha Fatima,Ilyas Khan,Sulaiman Bahadar,Abdul Qadeer,Mohamad S. Hakim. 2025

作者其他论文 更多>>