数字农科院2.0

Comprehensive Analysis Of The Molecular Characterization Of Gm Rice G6H1 Using A Paired-End Sequencing Approach

文献类型： 外文期刊

作者： Cui, Jinjie;Qu, Zhan;Wang, Canhua;Yang, Litao;Yang, Litao;Zhang, Yuchen;Cui, Jinjie;Zhang, Hanwen;Zhang, Xiujie

作者机构：

关键词： Molecular Characterization; Paired-End Sequencing; Dna Integration; Flanking Sequence; Copy Number; Unintended Integration; G6H1 Rice

期刊名称： FOOD CHEMISTRY

ISSN： 0308-8146

年卷期： 2020 年 309 卷

页码：

摘要： Molecular characterization of exogenous DNA integrations in host genome is a key aspect in risk assessment of bioengineered crops. However, gaining a clear understanding of the molecular characters of a bioengineered crop using conventional techniques remains a challenging task. Herein, we report the full molecular characterization of one new transgenic rice event G6H1 via a paired-end sequencing approach and bioinformatics analysis pipelines. Also, the molecular characterization reported was validated using conventional PCR, Sanger sequencing, and digital PCR. The results showed there is only one copy of the exogenous DNA inserted, which is located within chromosome 7 of the G6H1 genome. There is no other unintended integration of sequences from the transformation plasmid. These results indicated that the paired-end sequencing approach, combined with bioinformatics pipeline developed, is well suited to elucidate the molecular characteristics of bioengineered crops, and is efficient, low cost, and comprehensive.

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