数字农科院2.0

Using CRISPR-Cas9 to generate semi-dwarf rice lines in elite landraces

文献类型： 外文期刊

作者： Xingming Hu ; Yongtao Cui +; Guojun Dong +; Anhui Feng ; Danying Wang ; Chunyan Zhao ; Yu Zhang ; Jiang Hu ; Dali Zeng ; Longbiao Guo ; Qian Qian *

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期刊名称： Scientific Reports

ISSN： 0068-1261

年卷期： 2019 年 9 卷

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摘要： Genetic erosion refers to the loss of genetic variation in a crop. In China, only a few original landraces of rice (Oryza sativa) were used in breeding and these became the primary genetic background of modern varieties. Expanding the genetic diversity among Chinese rice varieties and cultivating high-yielding and high-quality varieties with resistance to different biotic and abiotic stresses is critical. Here, we used the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein9(Cas9) genome editing system to edit Semi-Dwarfl (SD1) in the elite landraces Kasalath and TeTePu (TTP), which contain many desired agronomic traits such as tolerance to low phosphorous and broad-spectrum resistance to several diseases and insects. Mutations of SD1 confer shorter plant height for better resistance to lodging. Field trials demonstrated that the yield of the new Kasalath and TTP mutant lines was better than that of the wild type under modern cultivation and that the lines maintained the same desirable agronomic characteristics as their wild-type progenitors. Our results showed that breeding using available landraces in combination with genomic data of different landraces and gene-editing techniques is an effective way to relieve genetic erosion in modern rice varieties.

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