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Toll-Like Receptor 2 (Tlr2) And Tlr4 Mediate The Iga Immune Response Induced By Mycoplasma Hyopneumoniae

文献类型： 外文期刊

作者： Li, X; Zhang, YK; Yin, B; Liang, JB; Jiang, F; Wu, WX

作者机构：

关键词： Mycoplasma Hyopneumoniae; Iga; Dc; B Cells; Tlr

期刊名称： INFECTION AND IMMUNITY

ISSN： 0019-9567

年卷期： 2020 年 88 卷 1 期

页码：

收录情况： JCR（2021版）

摘要： IgA plays an important role in mucosal immunity against infectious pathogens; however, the molecular mechanism of IgA secretion in response to infection remains largely unknown, particularly in Mycoplasma spp. In this study, we found that the levels of IgA in the peripheral blood serum, bronchoalveolar lavage fluid, nasal mucosa, trachea, hilar lymph nodes, and lung tissues of pigs increased significantly after infection with Mycoplasma hyopneumoniae. Furthermore, IgA and CD11c were detected in the lungs and hilar lymph nodes by immunohistochemical analysis, and colocalization of these two markers indicates that CD11c(+) cells play an important role in IgA mucosal immunity induced by M. hyopneumoniae. To investigate the regulatory mechanism of IgA, we separated mouse dendritic cells (DCs) from different tissues and mouse macrophages from the lungs and then cultured mouse B cells together with either DCs or macrophages in vitro. In the mouse lungDC/B (LDC/B) cell coculture, IgA secretion was increased significantly after the addition of whole-cell lysates of M. hyopneumoniae. The expression of both Toll-like receptor 2 (TLR2) and TLR4 was also upregulated, as determined by mRNA and protein expression analyses, whereas no obvious change in the expression of TLR3 and TLR7 was detected. Moreover, the IgA level decreased to the same as the control group when TLR2 or TLR4 was inhibited instead of TLR8 or TLR7/9. In conclusion, M. hyopneumoniae can stimulate the response of IgA through TLR2 and TLR4 in a mouse LDC/B cell coculture model, and the coculture model is an ideal tool for studying the IgA response mechanism, particularly that with Mycoplasma spp.

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