数字农科院2.0

Low Doses Of Carbendazim And Chlorothalonil Synergized To Impair Mouse Spermatogenesis Through Epigenetic Pathways

文献类型： 外文期刊

作者： Li, HT; Zhang, PF; Zhao, Y; Zhang, HF

作者机构：

关键词： Carbendazim; Chlorothalonil; Spermatogenesis; Epigenetic Signalling Pathways

期刊名称： ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY

ISSN： 0147-6513

年卷期： 2020 年 188 卷

页码：

收录情况： JCR（2021版）

摘要： Pesticides have been extensively produced and used to help the agricultural production which leads to the contamination of the environment, soil, groundwater sources, and even foodstuffs. Fungicides carbendazim (CBZ) and chlorothalonil (Chl) are widely applied in agriculture and other aspects. CBZ or Chl have been reported to disrupt spermatogenesis and decrease semen quality. However, it is not understood the effects of pubertal exposure to low doses of CBZ and Chl together, and the underlying mechanisms. Therefore, the aim of current investigation was to explore the negative impacts of pubertal exposure to low doses of CBZ and Chl together on spermatogenesis and the role of epigenetic modifications in the process. We demonstrated that CBZ and Chl together synergize to decrease sperm motility in vitro (CBZ 1.0 + Chl 0.1, CBZ 10.0 + CH1 1.0, CBZ 100.0 + Chl 10 mu M in incubation medium for 24 h) and sperm concentration and motility in vivo with ICR mice (CBZ 0.1 + Chl 0.1, CBZ 1.0 + CH1 1.0, CBZ 10.0 + Chl 10 mg/kg body weight; oral gavage for five weeks). CBZ + Chl significantly increase reactive oxygen species (ROS) and apoptosis by the increase in the protein level of caspase 8 in vitro. Moreover, CBZ + Chl synergized to disrupt mouse spermatogenesis with the disturbance in sperm production proteins and sperm proteins (VASA, A-Myb, STK31, AR, Acrosin). CBZ + Chl synergized to decrease the protein level of estrogen receptor alpha and the protein level of DNA methylation marker 5 mC in Leydig cells, and to increase the protein levels of histone methylation marker H3K9 and the methylation enzyme G9a in germ cells. Therefore, greater attention should be paid to the use of CBZ and Chl as pesticides to minimise their adverse impacts on spermatogenesis.

分类号：

  • 相关文献

[1]Low Dose Carbendazim Disrupts Mouse S.permatogenesis Might Be Through E strogen Receptor Related Histone And Dna Methylation. Liu, J, Zhang, PF, Zhao, Y, Zhang, HF. 2019

[2]匍柄霉属真菌对多菌灵耐药性分子机制的初步研究. 黄大野,李宝聚,石延霞,谢学文. 2013

[3]The Impacts Of Chlorothalonil And D.iflubenzuron On Apis Mellifera L . Larvae Reared In Vitro. Dai, PL, Jack, CJ, Mortensen, AN, Bloomquist, JR, Ellis, JD. 2018

[4]Simultaneous Lateral Flow Immunoassay For M.ulti-Class Chemical Contaminants In M aize And Peanut With One-Stop Sample Preparation. Wang, D, Zhu, JG, Zhang, Z, Zhang, Q, Zhang, W, Yu, L, Jiang, J, Chen, XM, Wang, XF, Li, PW. 2019

[5]Label-Free Based Quantitative Proteomic Analysis I.dentifies Proteins Involved In T he Testis Maturation Of Bactrocera Dorsalis (Hendel). Zhang, Meng-Yi,Gu, Pei-Ming,Smagghe, Guy,Smagghe, Guy,Smagghe, Guy,Wang, Jin-Jun,Wang, Jin-Jun,Wei, Dong,Wei, Dong,Wei, Dong,Zhang, Meng-Yi. 2018

[6]Molecular Cloning And Characterization Of Sycp3 And Tseg2 Genes In The Testicles Of Sexually Mature And Immature Yak. Ma, Xiaoming,Ahmad, Anum Ali,Yan, Ping,Zhang, Renzheng,Ahmad, Anum Ali,Chu, Min,Xie, Jianpeng,Ding, Xuezhi,Kalwar, Qudratullah,Ma, Fulong,Wu, Xiaoyun,Bao, Pengjia,Kalwar, Qudratullah. 2019

[7]Male Fertility Potential Molecular Mechanisms R.evealed By Itraq-Based Quantitative P roteomic Analysis Of The Epididymis From Wip1(-/-) Mice. Niu, PX, Wei, YH, Gao, Q, Zhang, X, Hu, YQ, Qiu, YQ, Mu, YL, Li, K. 2019

[8]Decrease In Male Mouse Fertility B.y Hydrogen Sulfide And/Or A mmonia Can Be Inheritable. Zhang, WD, Zhao, Y, Zhang, PF, Hao, YA, Yu, S, Min, LJ, Li, L, Ma, DX, Chen, L, Yi, B, Tang, XF, Meng, QS, Liu, L, Wang, SK, Shen, W, Zhang, HF. 2018

[9]Estrogen Receptor-Related Dna And Histone M.ethylation May Be Involved I n The Transgenerational Disruption In Spermatogenesis By Selective Toxic Chemicals. Han, X, Zhang, PF, Shen, W, Zhao, Y, Zhang, HF. 2019

[10]Alginate Oligosaccharides Improve Germ Cell Development And Testicular Microenvironment To Rescue Busulfan Disrupted Spermatogenesis. Zhao, Y, Zhang, PF, Ge, W, Feng, YN, Li, L, Sun, ZY, Zhang, HF, Shen, W. 2020

[11]The Effects Of Igf-1 On M.ouse Spermatogenesis Using An O rgan Culture Method. Yao, JF, Zuo, HY, Gao, J, Wang, MM, Wang, D, Li, XD. 2017

[12]Low Dose Chlorothalonil Impairs Mouse S.permatogenesis Through The Intertwining O f Estrogen Receptor Pathways With Histone And Dna Methylation. Zhang, PF, Zhao, Y, Zhang, HF, Liu, J, Feng, YN, Yin, S, Cheng, SF, Sun, XF, Min, LJ, Li, L, Shen, W. 2019

[13]Gestational Exposure To Low-Dose Zearalenone D.isrupting Offspring Spermatogenesis Might B e Through Epigenetic Modifications. Men, YH, Zhao, Y, Zhang, PF, Zhang, HF, Gao, YS, Liu, J, Feng, YN, Li, L, Shen, W, Sun, ZY, Min, LJ. 2019

[14]Identification Of Key Genes Associated With Spermatogenesis Arrest In Fox Hybrids Using Weighted Gene Co-Expression Network Analysis. Yang, TA, Liu, HM, Yang, YH, Hu, PF, Song, XC, Peng, YH, Yang, FH. 2020

[15]Pubertal Exposure To Low Doses O.f Zearalenone Disrupting Spermatogenesis T hrough Er Alpha Related Genetic And Epigenetic Pathways. Gao, YS, Zhao, Y, Zhang, HF, Zhang, PF, Liu, J, Feng, YN, Men, YH, Li, L, Shen, W, Sun, ZY, Min, LJ. 2019

[16]Sensitivity of Corynespora cassiicola to Carbendazim and Diethofencarb. Huang, Daye,Wang, Weiping,Shi, Yanxia,Xie, Xuewen,Li, Baoju.

[17]Complexation of synthetic CDM-AM copolymer with natamycin and carbendazim to improve solubility and fungicidal activity. Li, Yong-Fu,Ji, Jing,Guo, Qin,Ha, Yi-Ming,Li, Qing-Peng,Li, Yong-Fu,Ji, Jing,Guo, Qin,Ha, Yi-Ming,Li, Qing-Peng.

[18]Effect of household canning on the distribution and reduction of thiophanate-methyl and its metabolite carbendazim residues in tomato. Dong, Fengshou,Liu, Xingang,Xu, Jun,Li, Yuanbo,Han, Yongtao,Zhu, Yulong,Cheng, Youpu,Chen, Zenglong,Tao, Yan,Zheng, Yongquan,Liu, Na,Cheng, Youpu. 2014

[19]The transcription cofactor FgSwi6 plays a role in growth and development, carbendazim sensitivity, cellulose utilization, lithium tolerance, deoxynivalenol production and virulence in the filamentous fungus Fusarium graminearum. Jiang, Linghuo,Fan, Feiyu,Qiu, Dewen. 2013

[20]A Competitive Assay Based on Dual-Mode Au@Pt-DNA Biosensors for On-Site Sensitive Determination of Carbendazim Fungicide in Agricultural Products. Ge Chen,Rongqi Zhai,Guangyang Liu,Xiaodong Huang,Kaige Zhang,Xiaomin Xu,Lingyun Li,Yanguo Zhang,Jing Wang,Maojun Jin,Donghui Xu,A. M. Abd El-Aty. 2022

作者其他论文 更多>>