数字农科院2.0

Chalcone Synthase-Encoding Aechs Is Involved In Normal Petal Coloration In Actinidia Eriantha

文献类型： 外文期刊

作者： Li, YK; Cui, W; Qi, XJ; Qiao, CK; Lin, MM; Zhong, YP; Hu, CG; Fang, JB

作者机构：

关键词： Anthocyanin; Infiltration Grafting; Structural Gene; Petal Coloration; Actinidia Eriantha

期刊名称： GENES

ISSN：

年卷期： 2019 年 10 卷 12 期

页码：

收录情况： JCR（2021版）

摘要： Studies on anthocyanin biosynthesis have been mainly concentrated on the fruit, whereas few have focused the mechanism of flower coloration in kiwifruit. Here, we report that the structural gene, AeCHS, is involved in anthocyanin accumulation and indispensable for normal petal coloration in Actinidia eriantha. Petals from three different species including Actinidia eriantha (red petals), Actinidia hemsleyana (light pink petals) and Actinidia arguta (white petals) were selected for anthocyanin determination and gene expression analysis. The anthocyanin components in A. eriantha were significantly higher than in A. hemsleyana or A. arguta. Consistently, gene expression profiles suggested that AeCHS expression in A. eriantha was higher than in A. hemsleyana or A. arguta. Cluster analysis showed that AeCHS was clustered into a single group and distinctly separated from other genes, indicating the expression pattern of AeCHS gene was different from any other. Additionally, correlation analysis revealed AeCHS expression significantly correlated with anthocyanin content. The complete coding sequence of AeCHS was cloned from petals of A. eriantha 'Zaoxu', showing the length of AeCHS was 1170 bp encoding a protein of 389 amino acids. AeCHS was located in the cytoplasm, indicating it is indeed a structural gene involved in anthocyanin biosynthesis. AeCHS silencing performed by infiltration grafting-mediated virus-induced gene silencing (VIGS) reduced petal anthocyanin content and bleached red petals in A. eriantha. Our results confirm a crucial role of AeCHS in anthocyanin biosynthesis and accumulation in A. eriantha petals; furthermore, they offer important basic information and constitute a reference point for further research.

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