数字农科院2.0

The Collection Method for Crop Fungal Spores Based on an Efficient Microfluidic Device

文献类型: 外文期刊

作者: Pan Wang ; Shouqi Yuan *; Ning Yang *; Aiying Wang ; Alexander Fordjour ; Shengbo Chen

作者机构:

关键词: Crop Fungal Spores; Microfluidics; Separation Performance; Pretreatment

期刊名称: AEROSOL AND AIR QUALITY RESEARCH

ISSN: 1680-8584

年卷期: 2020 年 20 卷 1 期

页码:

收录情况: JCR(2021版) ; EI(2021版)

摘要: Nowadays, the airborne fungal spores have always taken an important role in the spread of crop fungal diseases and caused great concern. In this study, a novel efficient microfluidic chip for enriching the airborne fungal spores directly from gas flow was developed, which has better portability and cost-efficiency. The chip consists of three parts: half-wave pretreatment channel, inertial impactor, and low-pressure collection chamber. During the collection process, the particles were arranged in the radial position of the half-wave pretreatment channel based on their sizes, then separated by the inertial impactor and collected in the low-pressure collection chamber. The size distribution of the collected target was calculated by image process and recognition. The results show that the cut-off sizes of the proposed microfluidic device was found to be 4.83 mu m (first-stage) and 0.98 mu m (second-stage), respectively. The sharpness of the first and the second-stage collection efficiency curves were 1.31 and 1.79 respectively. The bounce effects and re-entrainment of particles can be eliminated by the low-pressure collection chamber without any silicon oil or grease. The collection reproducibility is acceptable. As a result, the proposed microfluidic chip can realize the crop fungal spore collection efficiently and can be used to improve the development of realtime crop fungal diseases monitoring technology.

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