数字农科院2.0

1-Hydroxyanthraquinone Exhibited Antibacterial Activity By Regulating Glutamine Synthetase Of Staphylococcus Xylosus As A Virulence Factor

文献类型： 外文期刊

作者： Wang, JP; Qu, QW; Liu, X; Cui, WQ; Yu, F; Chen, XR; Xing, XX; Zhou, YH; Yang, YB; Bello-Onaghise, G; Chen, XY; Li, XB; Li, YH

作者机构：

关键词： Bovine Mastitis; Staphylococcus Xylosus; Glutamine Synthetase; Virulence Factor; 1-Hydroxyanthraquinone; Antibacterial Activity

期刊名称： BIOMEDICINE & PHARMACOTHERAPY

ISSN： 0753-3322

年卷期： 2020 年 123 卷

页码：

收录情况： JCR（2021版）

摘要： Staphylococcus xylosus (S. xylosus) is one of the emerging pathogens causing bovine mastitis with high rate of isolation in most of the reported clinical and field cases. To verify the role of glutamine synthetase (GS) in the pathogenesis of S. xylosus, we evaluated the virulence level of the wild-type strain and its glnA mutant strain in biofilm assays in vitro and murine infection model in vivo. From the results, it was observed that the glnA mutant strain was attenuated and could reduce tissue damage. 1-Hydroxyanthraquinone (1-HAQ) is a kind of anthraquinones, it exhibited a significant inhibitory effect on the growth of S. xylosus and biofilm formation in vitro and provided anti-inflammatory effects in vivo. In addition, the rate at which it inhibits the biofilm, inflammatory factors, and CFU of wild-type strains were significantly higher than that of the mutant strains, indicating that 1-hAQ might have pharmacological effects against S. xylosus through the regulation of GS protein. The effect of 1-hAQ on GS was further confirmed by the down-regulation of glnA expression, reduced GS activity, Gln content and the results of molecular docking. Taken together, these findings suggest that 1-hAQ facilitated a significant attenuation of S. xylosus pathogenicity by regulating the GS protein: a vital virulence factor. Therefore, it can be inferred that 1-hAQ may serve as a potential source of organic compound for the development of novel alternative drugs in mitigating the menace of bovine mastitis.

分类号：

  • 相关文献

[1]Resistance response to Arenicin derivatives in Escherichia coli. Wang Z., Yang N., Teng D., Hao Y., Li T., Han H., Mao R., Wang J.. 2022

[2]Characterization of Shiga toxin-producing Escherichia coli isolated from Cattle and Sheep in Xinjiang province, China, using whole-genome sequencing. Liu Y., Li H., Chen X., Tong P., Zhang Y., Zhu M., Su Z., Yao G., Li G., Cai W.. 2022

[3]Comparative Proteomic Analysis Provides Insight I.nto The Key Proteins A s Possible Targets Involved In Aspirin Inhibiting Biofilm Formation Of Staphylococcus Xylosus. Xu, CG, Yang, YB, Zhou, YH, Hao, MQ, Ren, YZ, Wang, XT, Chen, JQ, Muhammad, I, Wang, S, Liu, D, Li, XB, Li, YH. 2017

[4]Reduced Glutamine Synthetase Activity Alters T.he Fecundity Of Female B actrocera Dorsalis (Hendel). Zhang, Su:Yun,Zhang, Su:Yun,Smagghe, Guy,Zhang, Meng:Yi,Wang, Jin:Jun,Wei, Dong,Zhang, Ying:Xin,Zhang, Meng:Yi,Smagghe, Guy,Wang, Jin:Jun,Smagghe, Guy,Wei, Dong,Zhang, Ying:Xin. 2019

[5]Itraq-Based Proteomic Analysis Provides Insights Into The Biological Mechanism Of Ammonium Metabolism In Tea Plant (Camellia Sinensis L.). Liu, MY, Tang, DD, Zhang, QF, Ruan, JY. 2020

[6]Comparative Genomics Analysis Of Mycoplasma Capricolum Subsp. Capripneumoniae 87001. Li, Y, Wang, R, Sun, WJ, Song, ZQ, Bai, F, Zheng, HJ, Xin, JQ. 2020

[7]Genome-Wide Identification And Expression Analysis O.f Cutinase Gene Family I n Rhizoctonia Cerealis And Functional Study Of An Active Cutinase Rccut1 In The Fungal Wheat Interaction. Lu, L, Rong, W, Massart, S, Zhang, ZY. 2018

[8]Genome-Wide Analysis of the First Sequenced Mycoplasma capricolum subsp capripneumoniae Strain M1601. . 2017

[9]Suppression Of Hyaluronidase Reduces Invasion And Establishment Ofhaemonchus Contortuslarvae In Sheep. Yang, XS, Khan, S, Zhao, XC, Zhang, JY, Nisar, A, Feng, XA. 2020

[10]Characterization Ofbacillus Cereusin Dairy Products In China. Liu, XY, Hu, Q, Xu, F, Ding, SY, Zhu, K. 2020

[11]Synthesis And Biological Activity Evaluation O.f Novel Heterocyclic Pleuromutilin D erivatives. Qin, Wenwen,Shang, Ruofeng,Yi, Yunpeng,Liu, Yu,Dong, Pengcheng,Fu, Yunxing,Liang, Jiangping. 2017

[12]Synthesis And Antibacterial Activities Of N.ovel Pleuromutilin Derivatives With A Substituted Pyrimidine Moiety. Liang, Jianping,Huang, Xin,Yi, Yunpeng,Yi, Yunpeng,Shang, Ruofeng,Liu, Yu,Huang, Xin,Xu, Ximing,Xu, Shuijin,Shang, Ruofeng,Liu, Yu,Liang, Jianping. 2017

[13]Cooperative Reinforcement Of Ionic Liquid A.nd Reactive Solvent On E nzymatic Synthesis Of Caffeic Acid Phenethyl Ester As An In Vitro Inhibitor Of Plant Pathogenic Bacteria. Wu, Fu-An,Wu, Fu-An,Xiao, Wei,Xu, Yan,Xu, Yan,Wang, Jun,Sheng, Sheng,Liu, Xi,Sheng, Sheng,Wang, Jun,Wang, Chao. 2017

[14]Antibacterial Activity And Pharmacokinetic Profile O.f A Promising Antibacterial A gent: 14-O-[(4-Amino-6-Hydroxy-Pyrimidine-2-Yl)Thioacetyl] Mutilin. Shang, Ruofeng,Pu, Wanxia,Yi, Yunpeng,Zhang, Chao,Fu, Yunxing,Liang, Jianping. 2018

[15]Improved Antibacterial Effects Of Alkali-Transformed S.aponin From Quinoa Husks A gainst Halitosis-Related Bacteria. Sun, XY, Yang, XS, Xue, P, Zhang, ZG, Ren, GX. 2019

[16]Characterization Of Subtilin L-Q11, A N.ovel Class I Bacteriocin S ynthesized By Bacillus Subtilis L-Q11 Isolated From Orchard Soil. Qin, YX, Wang, Y, He, YH, Zhang, Y, She, QX, Chai, YR, Lp, PL, Shang, QM. 2019

[17]Recent Advances In Antibacterial And A.ntiendotoxic Peptides Or Proteins F rom Marine Resources. Wang, ZL, Wang, XM, Wang, JH. 2018

[18]Synthesis And Biological Evaluation Of N.ovel Thioether Pleuromutilin Derivatives. Mu, SH, Liu, HX, Zhang, LF, Wang, XY, Xue, FQ, Zhang, Y. 2017

[19]An Approach For Searching Bioactive C.ompounds From Traditional Chinese P lant Food And Herb Medicines By Investigating Spectrum-Effect Relationships. Zhang, X, Zhang, LF, Cai, CY, Xue, FQ, Xiao, S. 2017

[20]Anti-Phytopathogenic And Cytotoxic Activities Of C.rude Extracts And Secondary M etabolites Of Marine-Derived Fungi. Zhao, DL, Wang, D, Tian, XY, Cao, F, Li, YQ, Zhang, CS. 2018

作者其他论文 更多>>