数字农科院2.0

Recombinant Pseudorabies Virus Expressing E2 Of Classical Swine Fever Virus (Csfv) Protects Against Both Virulent Pseudorabies Virus And Csfv

文献类型： 外文期刊

作者： Tong, W; Zheng, H; Li, GX; Gao, F; Shan, TL; Zhou, YJ; Yu, H; Jiang, YF; Yu, LX; Li, LW; Kong, N; Tong, GZ; Li, JC

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期刊名称： ANTIVIRAL RESEARCH

ISSN： 0166-3542

年卷期： 2020 年 173 卷

页码：

收录情况： JCR（2021版）

摘要： Both classical swine fever (CSF) and pseudorabies are highly contagious, economically significant diseases of swine in China. Although vaccination with the C-strain against classical swine fever virus (CSFV) is widely carried out and severe outbreaks of CSF seldom occur in China, CSF is sporadic in many pig herds and novel sub-subgenotypes of CSFV endlessly emerge. Thus, new measures are needed to eradicate CSFV from Chinese farms. The emergence of a pseudorabies virus (PRV) variant also posed a new challenge for the control of swine pseudorabies. Here, the recombinant PRV strain JS-2012-Delta gE/gI-E2 expressing E2 protein of CSFV was developed by inserting the E2 expression cassette into the intergenic region between the gG and gD genes of the gE/gI-deletion PRV variant strain JS-2012-Delta gE/gI. The recombinant virus was stable when passaged in vitro. A single vaccination of JS-2012-Delta gE/gI-E2 via intramuscular injection fully protected against lethal challenges of PRV and CSFV. Vaccination of piglets with the recombinant JS-2012-Delta gE/gI-E2 in the presence of high levels of maternally derived antibodies (Abs) to PRV can provide partial protection against lethal challenge of CSFV. Vaccination of the recombinant PRV JS-2012-Delta gE/gI-E2 strain did not induce the production of Abs to the gE protein of PRV or to the CSFV proteins other than E2. Thus, JS-2012-Delta gE/gI-E2 appears to be a promising recombinant marker vaccine candidate against PRV and CSFV for the control and eradication of the PRV variant and CSFV.

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