数字农科院2.0

Detection And Molecular Characterization Of A Phytoplasma In Eclipta Prostrata In China

文献类型: 外文期刊

作者: Chen, W; Li, Y; Fang, XP

作者机构:

关键词: Phytoplasma; Eclipta Prostrata; Candidatus Phytoplasma Aurantifolia

期刊名称: JOURNAL OF GENERAL PLANT PATHOLOGY

ISSN: 1345-2630

年卷期: 2020 年 86 卷 1 期

页码:

收录情况: JCR(2021版)

摘要: Witches' broom and phyllody, associated with phytoplasma infection, were observed on Eclipta prostrata, a common herb throughout the world, in Haikou, Hainan Province, China. Phytoplasma infection was confirmed using polymerase chain reaction and phytoplasma-specific primer pairs P1/P7, R16F2n/R16R2, rp(II)F/rp(II)R and subsequent amplicon sequencing. Based on the partial sequence of 16S rRNA gene and ribosomal protein gene, phylogenetic tree analysis and RFLP analysis of phytoplasma showed that the phytoplasma was a strain related to 'Candidatus Phytoplasma aurantifolia'. To our knowledge, this is the first report of a phytoplasma in E. prostrata worldwide.

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