数字农科院2.0

Marker-Assisted Selection In A Global Barley (Hordeum Vulgare Subsp. Vulgare) Collection Revealed A Unique Genetic Determinant Of The Naked Barley Controlled By The Nud Locus

文献类型： 外文期刊

作者： Lei, MM; Ali, M; Jiang, CC; Shen, ZH; Cai, Y; Yang, P; Feng, ZY

作者机构：

关键词： Cultivated Barley; Hulled Barley; Naked Barley; Nud; Marker Assisted Selection (Mas)

期刊名称： GENETIC RESOURCES AND CROP EVOLUTION

ISSN： 0925-9864

年卷期： 2020 年 67 卷 2 期

页码：

收录情况： JCR（2021版）

摘要： The cultivated barley (Hordeum vulgare subsp. vulgare) is classified into two categories according to the adherence of caryopsis, hulled or naked barley. The hulled barley is often used for animal feeding and brewing industries, while the naked barley is desired for human food consumption. The naked trait was reported to be controlled by a 17-kb deletion harboring the entire ethylene response factor (ERF) gene on the nud locus that resides on the barley chromosome 7HL. To rapidly select the hulled and naked barley accessions, here we developed a new PCR-based diagnostic marker which produces smaller product with an improved resolution if relative to the former one. Considering that (1) there were recently reports revealed the existence of additional loci that control the adherence of caryopsis in cultivated barley, (2) the origin of naked genotype remains not yet conclusive, we applied the newly developed marker to carry out a screening of the nud locus among the globally-collected 1291 barley accessions. The genotyping result was fully coincidence with the caryopsis phenotype. 98.9% of naked barley accessions were derived from East Asia, and rare of accessions without hulls from other geographic places were found. Thus, our results suggested that the 17-kb deletion of barley ERF gene fully explained the compromised formation of adhesive caryopsis in this collection, and this trait can be selected with the newly-generated co-dominant diagnostic marker.

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