数字农科院2.0

A Cucumber Green Mottle Mosaic Virus Vector For Virus-Induced Gene Silencing In Cucurbit Plants

文献类型： 外文期刊

作者： Liu, M; Liang, ZL; Aranda, MA; Hong, N; Liu, LM; Kang, BS; Gu, QS

作者机构：

关键词： Cucumber Green Mottle Mosaic Virus; Viral Vector; Virus-Induced Gene Silencing; Cucurbit Plants

期刊名称： PLANT METHODS

ISSN：

年卷期： 2020 年 16 卷 1 期

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收录情况： JCR（2021版）

摘要： Background Cucurbits produce fruits or vegetables that have great dietary importance and economic significance worldwide. The published genomes of at least 11 cucurbit species are boosting gene mining and novel breeding strategies, however genetic transformation in cucurbits is impractical as a tool for gene function validation due to low transformation efficiency. Virus-induced gene silencing (VIGS) is a potential alternative tool. So far, very few ideal VIGS vectors are available for cucurbits. Results Here, we describe a new VIGS vector derived from cucumber green mottle mosaic virus (CGMMV), a monopartite virus that infects cucurbits naturally. We show that the CGMMV vector is competent to induce efficient silencing of the phytoene desaturase (PDS) gene in the model plant Nicotiana benthamiana and in cucurbits, including watermelon, melon, cucumber and bottle gourd. Infection with the CGMMV vector harboring PDS sequences of 69-300 bp in length in the form of sense-oriented or hairpin cDNAs resulted in photobleaching phenotypes in N. benthamiana and cucurbits by PDS silencing. Additional results reflect that silencing of the PDS gene could persist for over two months and the silencing effect of CGMMV-based vectors could be passaged. Conclusions These results demonstrate that CGMMV vector could serve as a powerful and easy-to-use tool for characterizing gene function, controlling viral pathogens or even performing resistance breeding in cucurbits. Moreover, this study will possess considerable important reference value for developing different viral vectors.

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