数字农科院2.0

Electrochemical Antioxidant Screening And Evaluation Based On Guanine And Chitosan Immobilized Mos2 Nanosheet Modified Glassy Carbon Electrode (Guanine/Cs/Mos2/Gce)

文献类型： 外文期刊

作者： Tang, P; Tang, XS; Mei, SY; Xie, YX; Liu, LL; Ren, LC

作者机构：

关键词： Antioxidant Capacity; Chitosan; Guanine; Hydroxyl Radical; Mos2 Nanosheet

期刊名称： OPEN CHEMISTRY

ISSN： 2391-5420

年卷期： 2020 年 18 卷 1 期

页码：

收录情况： JCR（2021版）

摘要： In this study, an electrochemical biosensor based on guanine and chitosan immobilized MoS2 nanosheet modified glassy carbon electrode (guanine/CS/MoS2/GCE) was developed and employed for antioxidant screening and antioxidant capacity evaluation. The oxidation peak current of guanine was improved and nearly tripled after modifications of chitosan and MoS2 nanosheet. The immobilized guanine could be damaged by hydroxyl radicals generated in Fenton solution. However, in the presence of antioxidants, the guanine was protected and the oxidation peak current of guanine increased. This process mimics the mechanism of antioxidant protection in human body. The factors affecting preparation of sensor and detection of antioxidant capacity were optimized. At the optimum conditions, the guanine/CS/MoS2/GCE showed wide linear range, low detection limit, satisfactory reproducibility and stability for detection. Ascorbic acid was used as a model antioxidant to evaluate the antioxidant capacity. A good linearity was observed with a correlation coefficient of 0.9959 in the concentrations between 0.5 and 4.0 mg L-1. The antioxidant capacities of three flavonoids were also tested and the rank of antioxidant capacities was ascorbic acid (51.84%), quercetin (45.82%), fisetin (34.39%) and catechin (16.99%). Due to the rapid measurement and low cost, this sensor could provide an available sensing platform for antioxidant screening and evaluation.

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