数字农科院2.0

Development Of Genome-Wide Polymorphic Microsatellite Markers For Trichinella Spiralis

文献类型： 外文期刊

作者： Li, TT; Tang, B; Bai, X; Wang, XL; Luo, XN; Yan, HB; Zhu, HF; Jia, H; Liu, XL; Liu, MY

作者机构：

关键词： Trichinella Spiralis; Microsatellite; Cross-Amplification

期刊名称： PARASITES & VECTORS

ISSN： 1756-3305

年卷期： 2020 年 13 卷 1 期

页码：

收录情况： JCR（2021版）

摘要： Background Trichinella nematodes are globally distributed food-borne pathogens, in which Trichinella spiralis is the most common species in China. Microsatellites are a powerful tool in population genetics and phylogeographic analysis. However, only a few microsatellite markers were reported in T. spiralis. Thus, there is a need to develop and validate genome-wide microsatellite markers for T. spiralis. Methods Microsatellites were selected from shotgun genomic sequences using MIcroSAtellite identification tool (MISA). The identified markers were validated in 12 isolates of T. spiralis in China. Results A total of 93,140 microsatellites were identified by MISA from 9267 contigs in T. spiralis genome sequences, in which 16 polymorphic loci were selected for validation by PCR with single larvae from 12 isolates of T. spiralis in China. There were 7-19 alleles per locus (average 11.25 alleles per locus). The observed heterozygosity (H-O) and expected heterozygosity (H-E) ranged from 0.325 to 0.750 and 0.737 to 0.918, respectively. The polymorphism information content (PIC) ranged from 0.719 to 0.978 (average 0.826). Among the 16 loci, markers for 10 loci could be amplified from all 12 international standard strains of Trichinella spp. Conclusions Sixteen highly polymorphic markers were selected and validated for T. spiralis. Primary phylogenetic analysis showed that these markers might serve as a useful tool for genetic studies of Trichinella parasites.

分类号：

  • 相关文献

[1]Association Mapping Reveals Genetic Loci A.ssociated With Important Agronomic T raits In Lentinula Edodes, Shiitake Mushroom. Nong, Wenyan,Li, Chuang,Xiao, Yang,Zhang, Lin,Yang, Zhiquan,Cheung, Man-Kit,Bian, Yinbing,Gong, Wenbing,Kwan, Hoi-Shan,Gong, Wenbing. 2017

[2]Mapping Molecular Diversity Of Indigenous G.oat Genetic Resources Of A sia. Periasamy, K, Vahidi, SMF, Silva, P, Faruque, MO, Naqvi, AN, Basar, M, Cao, JH, Zhao, SH, Thuy, LT, Pichler, R, Podesta, MG, Shamsuddin, M, Boettcher, P, Garcia, JF, Han, JL, Marsan, PA, Diallo, A, Viljoen, GJ. 2017

[3]Evaluation Of Genetic Diversity And S.tructure Of Vietnamese Goat P opulations Using Multi Locus Microsatellite Markers. Thuy, LT, Van Binh, D, Binh, NT, Minh, LQ, Thuy, TTT, Ton, ND, Van Ba, N, Han, JL, Periasamy, K. 2017

[4]Development Of Highly Informative Genome-Wide S.ingle Sequence Repeat Markers F or Breeding Applications In Sesame And Construction Of A Web Resource: Sisatbase. Dossa, K, Yu, JY, Liao, BS, Cisse, N, Zhang, XR. 2017

[5]Combined Analyses Of Chloroplast Dna H.aplotypes And Microsatellite Markers R eveal New Insights Into The Origin And Dissemination Route Of Cultivated Pears Native To East Asia. Yue, XY, Zheng, XY, Zong, Y, Jiang, S, Hu, CY, Yu, PY, Liu, GQ, Cao, YF, Hu, HJ, Teng, YW. 2018

[6]Genetic Diversity And Population Structure O.f Commelina Communis In C hina Based On Simple Sequence Repeat Markers. Yang Juan,Dong Jin-gao,Yang Juan,Yu Hai-yan,Li xiang-ju. 2018

[7]Current Genetic Diversity In Eight L.ocal Chinese Sheep Populations. Guang-Xin, E, Ma, YH, Chu, MX, Hong, QH, Huang, YF. 2019

[8]Genetic Diversity Estimation Of Yunnan I.ndigenous Goat Breeds Using M icrosatellite Markers. E, GX, Hong, QH, Zhao, YJ, Ma, YH, Chu, MX, Zhu, L, Huang, YF. 2019

[9]Genetic Diversity Analysis Of Major S.ri Lankan Goat Populations U sing Microsatellite And Mitochondrial Dna D-Loop Variations. Silva, P, Dematawewa, CMB, Kurukulasuriya, M, Utsunomiya, YT, Garcia, JF, Pichler, R, Thiruvenkadan, AK, Ramasamy, S, Han, JL, Periasamy, K. 2017

[10]Genetic Diversity Of The Chinese G.oat In The Littoral Z one Of The Yangtze River As Assessed By Microsatellite And Mtdna. E, GX, Zhao, YJ, Chen, LP, Ma, YH, Chu, MX, Li, XL, Hong, QH, Li, LH, Guo, JJ, Zhu, L, Han, YG, Gao, HJ, Zhang, JH, Jiang, HZ, Jiang, CD, Wang, GF, Ren, HX, Jin, ML, Sun, YZ, Zhou, P, Huang, YF. 2018

[11]Detection of anti-Trichinella antibodies in serum of experimentally-infected swine by immunochromatographic strip. Fu, B. Q.,Li, W. H.,Gai, W. Y.,Yao, J. X.,Qu, Z. G.,Xie, Z. Z.,Wang, Y. H.,Zhang, D. L.,Blaga, R.. 2013

[12]Identification of stage-specifically expressed genes of Trichinella spiralis by suppression subtractive hybridization. Liu, M. Y.,Wang, X. L.,Fu, B. Q.,Li, C. Y.,Wu, X. P.,Le Rhun, D.,Chen, Q. J.,Boireau, P.. 2007

[13]Study of the 49 kDa excretory-secretory protein gene of Trichinella nativa and Trichinella spiralis. Zheng, B. L.,Xiao, L. H.,Wang, X. R.,Li, D. M.,Lu, Y. X.,Zhang, Y.,Yan, Q. B.,Song, M. X.. 2007

[14]Cloning, identification, and bioinformatics analysis of a putative aquaporin TsAQP from Trichinella spiralis. Cui, J. M.,Zhang, N. Z.,Li, W. H.,Yan, H. B.,Fu, B. Q.,Fu, B. Q.. 2015

[15]Cloning and characterization of thioredoxin peroxidases from Trichinella spiralis. Zhang, N. Z.,Liu, J. Y.,Li, W. H.,Li, L.,Qu, Z. G.,Li, T. T.,Cui, J. M.,Yang, Y.,Jia, W. Z.,Fu, B. Q.,Jia, W. Z.,Fu, B. Q..

[16]Proteomic analysis of differentially expressed proteins in the three developmental stages of Trichinella spiralis. Liu, J. Y.,Zhang, N. Z.,Li, W. H.,Li, L.,Yan, H. B.,Qu, Z. G.,Li, T. T.,Cui, J. M.,Yang, Y.,Jia, W. Z.,Fu, B. Q.,Jia, W. Z.,Fu, B. Q..

[17]Use of mitochondrial RNA genes for the differentiation of four Trichinella species by multiplex PCR amplification. Blaga, R.,Fu, BaoQuan,Le Rhun, D.,Le Naour, E.,Heckman, A.,Zocevic, A.,Boireau, P.,Liu, MingYuan.

[18]High-level expression and characterization of two serine protease inhibitors from Trichinella spiralis. Zhang, Zhaoxia,Mao, Yixian,Li, Da,Zhang, Yvhan,Li, Wei,Li, Li,Lu, Yixin,Jia, Honglin,Zheng, Jun.

[19]Molecular characterization of a cathepsin F-like protease in Trichinella spiralis. Qu, Zi-gang,Ma, Xue-ting,Li, Wen-hui,Zhang, Nian-zhang,Yue, Long,Cui, Jian-min,Cai, Jian-ping,Jia, Wan-zhong,Fu, Bao-quan,Cai, Jian-ping,Jia, Wan-zhong,Fu, Bao-quan. 2015

[20]Dendritic Cells Treated By Trichinella S.piralis Muscle Larval Excretory/Secretory P roducts Alleviate Tnbs-Induced Colitis In Mice. Jin, XM, Yang, Y, Bai, X, Shi, HN, Zhang, WB, Zhang, ZZ, Jia, WZ, Lin, JJ, Liu, MY, Liu, XL. 2019

作者其他论文 更多>>